On-command on/off switching of progenitor cell and cancer cell polarized motility and aligned morphology via a cytocompatible shape memory polymer scaffold

Abstract In vitro biomaterial models have enabled advances in understanding the role of extracellular matrix (ECM) architecture in the control of cell motility and polarity. Most models are, however, static and cannot mimic dynamic aspects of in vivo ECM remodeling and function. To address this limi...

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Bibliographic Details
Published in:Biomaterials 2017-09, Vol.140, p.150-161
Main Authors: Wang, Jing, Quach, Andy, Brasch, Megan E, Turner, Christopher E, Henderson, James H
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
Biocompatible Materials - chemistry
Cell alignment
Cell Line
Cell Line, Tumor
Cell Movement
Cell Nucleus - ultrastructure
Cell Polarity
Cell Shape
Dentistry
Dynamic architectural change
Electrospun scaffold
Extracellular Matrix - chemistry
Humans
Mice
Polarized morphology
Polarized motility
Polymers - chemistry
Shape memory polymer
Stem Cells - cytology
Tissue Scaffolds - chemistry
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Summary:Abstract In vitro biomaterial models have enabled advances in understanding the role of extracellular matrix (ECM) architecture in the control of cell motility and polarity. Most models are, however, static and cannot mimic dynamic aspects of in vivo ECM remodeling and function. To address this limitation, we present an electrospun shape memory polymer scaffold that can change fiber alignment on command under cytocompatible conditions. Cellular response was studied using the human fibrosarcoma cell line HT-1080 and the murine mesenchymal stem cell line C3H/10T1/2. The results demonstrate successful on-command on/off switching of cell polarized motility and alignment. Decrease in fiber alignment causes a change from polarized motility along the direction of fiber alignment to non-polarized motility and from aligned to unaligned morphology, while increase in fiber alignment causes a change from non-polarized to polarized motility along the direction of fiber alignment and from unaligned to aligned morphology. In addition, the findings are consistent with the hypothesis that increased fiber alignment causes increased cell velocity, while decreased fiber alignment causes decreased cell velocity. On-command on/off switching of cell polarized motility and alignment is anticipated to enable new study of directed cell motility in tumor metastasis, in cell homing, and in tissue engineering.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2017.06.016