Selective tumor cell death induced by irradiated riboflavin through recognizing DNA G-T mismatch

Riboflavin (vitamin B2) has been thought to be a promising antitumoral agent in photodynamic therapy, though the further application of the method was limited by the unclear molecular mechanism. Our work reveals that riboflavin was able to recognize G-T mismatch specifically and induce single-strand...

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Bibliographic Details
Published in:Nucleic acids research 2017-09, Vol.45 (15), p.8676-8683
Main Authors: Yuan, Yi, Zhao, Yongyun, Chen, Lianqi, Wu, Jiasi, Chen, Gangyi, Li, Sheng, Zou, Jiawei, Chen, Rong, Wang, Jian, Jiang, Fan, Tang, Zhuo
Format: Article
Language:English
Subjects:
Base Pair Mismatch - drug effects
Base Sequence
Binding Sites - drug effects
Binding Sites - genetics
Cell Death - drug effects
Cell Death - radiation effects
Chemical Biology and Nucleic Acid Chemistry
DNA Damage - drug effects
HCT116 Cells
Humans
Light
Neoplasms - drug therapy
Neoplasms - pathology
Photochemotherapy - methods
Riboflavin - pharmacology
Riboflavin - radiation effects
Riboflavin - therapeutic use
Substrate Specificity - drug effects
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Summary:Riboflavin (vitamin B2) has been thought to be a promising antitumoral agent in photodynamic therapy, though the further application of the method was limited by the unclear molecular mechanism. Our work reveals that riboflavin was able to recognize G-T mismatch specifically and induce single-strand breaks in duplex DNA targets efficiently under irradiation. In the presence of riboflavin, the photo-irradiation could induce the death of tumor cells that are defective in mismatch repair system selectively, highlighting the G-T mismatch as potential drug target for tumor cells. Moreover, riboflavin is a promising leading compound for further drug design due to its inherent specific recognition of the G-T mismatch.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gkx602