PHF7, a novel male gene influences female fecundity and population growth in Nilaparvata lugens Stål (Hemiptera: Delphacidae)

PHF7 exhibits male-specific expression in early germ cells, germline stem cells and spermatogonia in insects, and its expression promotes spermatogenesis in germ cells when they are present in a male soma. However, the influence of male-specific PHF7 on female reproductive biology via mating remains...

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Bibliographic Details
Published in:Scientific reports 2017-09, Vol.7 (1), p.11611-13, Article 11611
Main Authors: Ge, Lin-Quan, Xia, Ting, Huang, Bo, Gu, Hao-Tian, Song, Qi-Sheng, Yang, Guo-Qing, Liu, Fang, Wu, Jin-Cai
Format: Article
Language:English
Subjects:
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Accessory gland
Animal reproduction
Arginine
Body weight
Fat body
Fecundity
Females
Gene expression
Germ cells
Hatching
Humanities and Social Sciences
Mating
multidisciplinary
Nilaparvata lugens
Oviposition
Population growth
RNA-mediated interference
Science
Science (multidisciplinary)
Seminal fluid
Spermatogenesis
Spermatogonia
Stem cells
Symbionts
Vitellogenin
Yeasts
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Summary:PHF7 exhibits male-specific expression in early germ cells, germline stem cells and spermatogonia in insects, and its expression promotes spermatogenesis in germ cells when they are present in a male soma. However, the influence of male-specific PHF7 on female reproductive biology via mating remains unclear. Thus, we investigated the potential impacts of male PHF7, existed in seminal fluid of Nilaparvata lugens ( Nl PHF7), on fecundity and population growth via mating. Our results revealed that suppressing male NlPHF7 expression by RNAi led to decreases in body weight, soluble accessory gland protein content, arginine content, and reproductive organ development in males, resulting in significant reduction of oviposition periods and fecundity in females, and significant decrease in body weight, fat body and ovarian protein content, yeast-like symbionts abundance, ovarian development and vitellogenin gene expression in their female mating partners. Similarly, suppression of NlPHF7 expression in males mated with the control female reduced population growth and egg hatching rate, but did not influence gender ratio. We infer that Nl PHF7 play a role important in stimulating female fecundity via mating. This study provides valuable information by identifying a potentially effective target gene for managing BPH population through RNAi.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-11524-2