Elongation of Axon Extension for Human iPSC-Derived Retinal Ganglion Cells by a Nano-Imprinted Scaffold

Optic neuropathies, such as glaucoma and Leber's hereditary optic neuropathy (LHON) lead to retinal ganglion cell (RGC) loss and therefore motivate the application of transplantation technique into disease therapy. However, it is a challenge to direct the transplanted optic nerve axons to the c...

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Bibliographic Details
Published in:International journal of molecular sciences 2017-09, Vol.18 (9), p.2013
Main Authors: Yang, Tien-Chun, Chuang, Jen-Hua, Buddhakosai, Waradee, Wu, Wen-Ju, Lee, Chen-Ju, Chen, Wun-Syuan, Yang, Yi-Ping, Li, Ming-Chia, Peng, Chi-Hsien, Chen, Shih-Jen
Format: Article
Language:English
Subjects:
Axon guidance
Axons
Axons - physiology
Biocompatibility
Cell culture
Cell Differentiation - physiology
Computer architecture
Elongation
Glaucoma
Grooves
Humans
Induced Pluripotent Stem Cells - physiology
Mimicry
Nanotechnology - methods
Neurites - physiology
Optic nerve
Optic neuropathy
Organs
Pluripotency
Retina
Retinal ganglion cells
Retinal Ganglion Cells - physiology
Stem cells
Time Factors
Tissue engineering
Tissue Engineering - methods
Tissue Scaffolds
Transplantation
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Summary:Optic neuropathies, such as glaucoma and Leber's hereditary optic neuropathy (LHON) lead to retinal ganglion cell (RGC) loss and therefore motivate the application of transplantation technique into disease therapy. However, it is a challenge to direct the transplanted optic nerve axons to the correct location of the retina. The use of appropriate scaffold can promote the proper axon growth. Recently, biocompatible materials have been integrated into the medical field, such as tissue engineering and reconstruction of damaged tissues or organs. We, herein, utilized nano-imprinting to create a scaffold mimicking the in vitro tissue microarchitecture, and guiding the axonal growth and orientation of the RGCs. We observed that the robust, long, and organized axons of human induced pluripotent stem cell (iPSC)-derived RGCs projected axially along the scaffold grooves. The RGCs grown on the scaffold expressed the specific neuronal biomarkers indicating their proper functionality. Thus, based on our in vitro culture system, this device can be useful for the neurophysiological analysis and transplantation for ophthalmic neuropathy treatment.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms18092013