Neupogen and mesenchymal stem cells are the novel therapeutic agents in regeneration of induced endometrial fibrosis in experimental rats

Endometrial fibrosis is the presence of intrauterine adhesions (IUAs) after any uterine surgery or curettage and it results in infertility and recurrent pregnancy loss. We evaluated the role of human mesenchymal stem cells (hMSCs) as a therapeutic agent of endometrial fibrosis. We also compared the...

Full description

Saved in:
Bibliographic Details
Published in:Bioscience reports 2017-10, Vol.37 (5)
Main Authors: Sabry, Dina, Mostafa, Abeer, Marzouk, Samar, Ibrahim, Walaa, Ali, Hanan H M, Hassan, Aymen, Shamaa, Ashraf
Format: Article
Language:English
Subjects:
Animals
Biomarkers - metabolism
Disease Models, Animal
Endometrium - pathology
Endometrium - physiology
Estrogens - administration & dosage
Estrogens - therapeutic use
Female
Fibrosis
Filgrastim - administration & dosage
Filgrastim - therapeutic use
Mesenchymal Stem Cell Transplantation
Mesenchymal Stromal Cells - metabolism
Rats
Rats, Wistar
Regeneration
Uterine Diseases - pathology
Uterine Diseases - therapy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Endometrial fibrosis is the presence of intrauterine adhesions (IUAs) after any uterine surgery or curettage and it results in infertility and recurrent pregnancy loss. We evaluated the role of human mesenchymal stem cells (hMSCs) as a therapeutic agent of endometrial fibrosis. We also compared the effect of MSCs with the effect of estrogen and neupogen either each alone or as a combined therapy with MSCs. This experimental study was performed on 84 albino rats which were divided into seven groups ( =12 rats/group) as follows, 1: normal control rats, 2: induced fibrosis, 3: induced fibrosis that received oral estrogen, 4: induced fibrosis that received hMSCs, 5: induced fibrosis that received hMSCs and estrogen, 6: induced fibrosis that received neupogen, and 7: induced fibrosis that received hMSCs and neupogen. The extent of fibrosis, vascularization, and inflammation were evaluated by; qRT-PCR for interleukin 1 (IL-1), interleukin 6 (IL-6), TNF, vascular endothelial growth factor (VEGF), transforming growth factor-β (TGF-β), and RUNX; ELISA for connective tissue growth factor (CTGF); Western blotting for collagen-I; immunohistochemistry examination for VEGF and RUNX-2; and histopathological assessment. In therapeutic groups either by hMSCs alone or combined with estrogen or neupogen; fibrosis and inflammation (IL-1, IL-6, TNF, TGF-β, RUNX, CTGF, and collagen-I) were significantly decreased but vascularization (VEGF) was significantly increased (
ISSN:0144-8463
1573-4935
DOI:10.1042/BSR20170794