Allelic analysis of low molecular weight glutenin subunits using 2-DGE in Korean wheat cultivars

Two-dimensional gel electrophoresis (2-DGE) was used as a complement to SDS-PAGE to determine the allelic compositions of LMW-GS in 32 Korean wheat cultivars. Protein patterns generated by 2-DGE from each cultivar were compared to patterns from standard wheat cultivars for each allele. At the Glu-A3...

Full description

Saved in:
Bibliographic Details
Published in:Breeding Science 2017, Vol.67(4), pp.398-407
Main Authors: Lee, Jong-Yeol, Jang, You-Ran, Beom, Hye-Rang, Altenbach, Susan B., Lim, Sun-Hyung, Lee, Choung Keun
Format: Article
Language:English
Subjects:
2-DGE
Alleles
allelic analysis
Cultivars
flour quality
Gel electrophoresis
Glutenin
LMW-GS
Loci
Low molecular weights
Molecular weight
Proteins
Sodium lauryl sulfate
Triticum
Triticum aestivum L
Wheat
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two-dimensional gel electrophoresis (2-DGE) was used as a complement to SDS-PAGE to determine the allelic compositions of LMW-GS in 32 Korean wheat cultivars. Protein patterns generated by 2-DGE from each cultivar were compared to patterns from standard wheat cultivars for each allele. At the Glu-A3 locus, thirteen c, twelve d, three e (null), two g and two new alleles were identified. At the Glu-B3 locus, one b, nineteen d, four h, one i and five ad alleles were identified. At the Glu-D3 locus, twenty-three a, four b, four c and one l alleles were identified. When compared to results obtained previously using SDS-PAGE, there were discrepancies in the allelic designations of 10 of 32 cultivars (31%). While SDS-PAGE is a rapid and relatively simple method for assessing LMW-GS composition, the similar mobilities of the proteins makes it difficult to discriminate certain alleles. 2-DGE is a more complicated technique, but provides a more accurate picture of the complement of the LMW-GS in a given cultivar. In addition to providing essential information for wheat breeders, the 2-DGE reference maps generated in this study will make it possible to study the contributions of individual LMW-GS to flour quality.
ISSN:1344-7610
1347-3735
DOI:10.1270/jsbbs.16106