SCDT-25. INSIGHT INTO THE CYTOTOXIC EFFECTS OF TUMOR INFILTRATING LYMPHOCYTES (TILs) TO CD133+ GLIOBLASTOMA STEM CELLS (GSCs)

• Published in: Neuro-oncology (Charlottesville, Va.) Va.), 2017-11, Vol.19 (suppl_6), p.vi270-vi270
• Main Authors: Lee, Han-Chung, Huang, Yin-Cheng, Chen, Yueh-Sheng, Yang, Wen-Kuang
• Format: Article
• Language: English
• Subjects: Abstracts
• ISSN: 1522-8517; 1523-5866
• DOI: 10.1093/neuonc/nox168.1108

Glioblastoma multiforme (GBM) is the most prevalent and high malignant primary brain tumors, and contains a small cohort of self-renewing, tumorigenic glioblastoma stem cells (GSCs) that contribute to tumor relapse and therapeutic resistance. GSCs are typically recognized by the expression of cell surface marker, CD133, encoded from prominin-1 ( PROM1 ) gene. Recent studies have indicated CD133 + cancer stem-like cells are enriched after the treatment of ionizing radiation (IR). To investigate the effect of cytotoxic TILs to glioblastoma cells experienced irradiation, we used post-vaccination TILs treat with glioblastoma cells derived from patient’s tissue specimens without experienced and experienced irradiation respectively. The effect of cytotoxic TILs was not evident in glioblastoma cells experienced IR until 120 hours later. To further investigate whether immune cytokines have cytotoxicity in CD133 + glioblastoma cells, we observed that TNF-α and IFN-γ have the cytotoxic effects in CD133 + glioblastoma cells, implicating the reason why the cytotoxic of CD8 + TILs were delayed to 120 hours. These data provide the framework for understanding the radioresistance in glioblastoma cells that can be used to develop an efficient vaccine.