Influenza virus‐like particles harboring H9N2 HA and NA proteins induce a protective immune response in chicken

• Published in: Influenza and other respiratory viruses 2017-11, Vol.11 (6), p.518-524
• Main Authors: Li, Xin, Ju, Houbin, Liu, Jian, Yang, Dequan, Qi, Xinyong, Yang, Xianchao, Qiu, Yafeng, Zheng, Jie, Ge, Feifei, Zhou, Jinping
• Format: Article
• Language: English
• Subjects: Adaptability; Animals; Antibodies; Antibodies, Viral - blood; Antigenicity; Avian flu; Avian influenza; Cell culture; Chickens; Electron microscopy; Epidemics; Exo-a-sialidase; Genotypes; H9N2; Hemagglutination inhibition; Hemagglutination Inhibition Tests; Hemagglutinin Glycoproteins, Influenza Virus - administration & dosage; Hemagglutinin Glycoproteins, Influenza Virus - chemistry; Hemagglutinin Glycoproteins, Influenza Virus - genetics; Hemagglutinins; Homology; Immune response; Immune system; Immunity; Immunization; Influenza; Influenza A Virus, H9N2 Subtype - chemistry; Influenza A Virus, H9N2 Subtype - genetics; Influenza A Virus, H9N2 Subtype - immunology; Influenza in Birds - immunology; Influenza in Birds - prevention & control; Influenza vaccines; Influenza Vaccines - administration & dosage; Influenza Vaccines - immunology; Influenza viruses; Medical research; Medicine, Experimental; Neuraminidase - administration & dosage; Neuraminidase - chemistry; Neuraminidase - genetics; Original; Pandemics; Poultry; Poultry Diseases - prevention & control; Poultry Diseases - virology; Proteins; Respiratory diseases; Specific Pathogen-Free Organisms; Subgroups; Vaccination - veterinary; vaccine; Vaccines; Vaccines, Inactivated - immunology; Vaccines, Virus-Like Particle - administration & dosage; Vaccines, Virus-Like Particle - immunology; Virus Shedding; Virus-like particles; Viruses
• ISSN: 1750-2640; 1750-2659
• DOI: 10.1111/irv.12472

Background Avian influenza viruses represent a growing threat of an influenza pandemic. The co‐circulation of multiple H9N2 genotypes over the past decade has been replaced by one predominant genotype—G57 genotype, which displays a changed antigenicity and improved adaptability in chickens. Effective H9N2 subtype avian influenza virus vaccines for poultry are urgently needed. Objective In this study, we constructed H9N2 subtype avian influenza virus‐like particle (VLP) and evaluated its protective efficacy in specific pathogen‐free (SPF) chickens to lay the foundation for developing an effective vaccine against influenza viruses. Methods Expression of influenza proteins in VLPs was confirmed by Western blot, hemagglutination inhibition (HI), and neuraminidase inhibition (NI). The morphology was observed by electron microscopy. A group of 15 three‐week‐old SPF chickens was divided into three subgroups of five chickens immunized with VLP, commercial vaccine, and PBS. Challenge study was performed to evaluate efficacy of VLP vaccine. Results and Conclusions The hemagglutinin (HA) and neuraminidase (NA) proteins were co‐expressed in the infected cells, self‐assembled, and were released into the culture medium in the form of VLPs of diameter ~80 nm. The VLPs exhibited some functional characteristics of a full influenza virus, including hemagglutination and neuraminidase activity. In SPF chickens, the VLPs elicited serum antibodies specific for H9N2 and induced a higher HI titer (as detected by a homologous antigen) than did a commercial H9N2 vaccine (A/chicken/Shanghai/F/1998). Viral shedding from VLP vaccine subgroup was reduced compared with commercial vaccine subgroup and control subgroup.