Single-cell RNA-seq reveals a distinct transcriptome signature of aneuploid hematopoietic cells

• Published in: Blood 2017-12, Vol.130 (25), p.2762-2773
• Main Authors: Zhao, Xin, Gao, Shouguo, Wu, Zhijie, Kajigaya, Sachiko, Feng, Xingmin, Liu, Qingguo, Townsley, Danielle M., Cooper, James, Chen, Jinguo, Keyvanfar, Keyvan, Fernandez Ibanez, Maria del Pilar, Wang, Xujing, Young, Neal S.
• Format: Article
• Language: English
• Subjects: Adult; Aneuploidy; Bone Marrow Cells; Bone Marrow Diseases - genetics; Bone Marrow Diseases - pathology; Case-Control Studies; Chromosome Deletion; Chromosome Disorders - genetics; Chromosomes, Human, Pair 7; Female; Gene Expression Regulation, Neoplastic; Hematopoietic Stem Cells; Humans; Male; Middle Aged; Myeloid Neoplasia; Sequence Analysis, RNA; Single-Cell Analysis - methods; Transcriptome - genetics
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood-2017-08-803353

Cancer cells frequently exhibit chromosomal abnormalities. Specific cytogenetic aberrations often are predictors of outcome, especially in hematologic neoplasms, such as monosomy 7 in myeloid malignancies. The functional consequences of aneuploidy at the cellular level are difficult to assess because of a lack of convenient markers to distinguish abnormal from diploid cells. We performed single-cell RNA sequencing (scRNA-seq) to study hematopoietic stem and progenitor cells from the bone marrow of 4 healthy donors and 5 patients with bone marrow failure and chromosome gain or loss. In total, transcriptome sequences were obtained from 391 control cells and 588 cells from patients. We characterized normal hematopoiesis as binary differentiation from stem cells to erythroid and myeloid-lymphoid pathways. Aneuploid cells were distinguished from diploid cells in patient samples by computational analyses of read fractions and gene expression of individual chromosomes. We confirmed assignment of aneuploidy to individual cells quantitatively, by copy-number variation, and qualitatively, by loss of heterozygosity. When we projected patients' single cells onto the map of normal hematopoiesis, diverse patterns were observed, broadly reflecting clinical phenotypes. Patients' monosomy 7 cells showed downregulation of genes involved in immune response and DNA damage checkpoint and apoptosis pathways, which may contribute to the clonal expansion of monosomy 7 cells with accumulated gene mutations. scRNA-seq is a powerful technique through which to infer the functional consequences of chromosome gain and loss and explore gene targets for directed therapy. •We distinguished aneuploid cells from diploid cells within the hematopoietic stem and progenitor cells using scRNA-seq.•Monosomy 7 cells showed downregulated pathways involved in immune response and maintenance of DNA stability. [Display omitted]