Anti‑inflammatory effect of quercetin and galangin in LPS‑stimulated RAW264.7 macrophages and DNCB‑induced atopic dermatitis animal models

Flavonols are compounds that have been shown to possess potent anti-inflammatory effects in cellular and animal models of inflammation. In the present study, the anti-inflammatory effects and mechanisms of two natural flavonols, quercetin and galangin, in lipopolysaccharide (LPS)-stimulated RAW264.7...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular medicine 2018-02, Vol.41 (2), p.888-898
Main Authors: Lee, Hae Nim, Shin, Seong Ah, Choo, Gang Sik, Kim, Hyeong Jin, Park, Young Seok, Kim, Byeong Soo, Kim, Sang Ki, Cho, Sung Dae, Nam, Jeong Seok, Choi, Chang Sun, Che, Jeong Hwan, Park, Byung Kwon, Jung, Ji Youn
Format: Article
Language:English
Subjects:
Apoptosis
Atopic dermatitis
Care and treatment
Dermatitis
Development and progression
Eczema
Experiments
Flavonoids
Fruits
Health aspects
Immunoglobulins
Inflammation
Kinases
Lymphatic system
Penicillin
Quercetin
Studies
Tumor necrosis factor-TNF
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Flavonols are compounds that have been shown to possess potent anti-inflammatory effects in cellular and animal models of inflammation. In the present study, the anti-inflammatory effects and mechanisms of two natural flavonols, quercetin and galangin, in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages were investigated. It was identified that quercetin and galangin markedly reduced the production of nitric oxide (NO), inducible NO synthase and interleukin-6, and the nuclear translocation of nuclear factor-[kappa]B (NF-[kappa]B). In addition, LPS-induced activation of extracellular signal-regulated kinase 1/2 (Erk1/2) and c-Jun N-terminal kinase (JNK) was suppressed by quercetin and galangin. Taken together, these data implied that NF-[kappa]B, Erk1/2 and JNK may be potential molecular targets of quercetin and galangin in an LPS-induced inflammatory response. Subsequently, the effects of oral administration of quercetin or galangin, either alone or in combination, in a 2,4-dinitrochlorobenzene-induced atopic dermatitis (AD) mouse model were investigated. As a result, measurements of ear thickness and the levels of serum immunoglobulin E, and histological analysis revealed that the two flavonols led to a decrease in inflammation, whereas, in combination, they were even more effective. These results suggested that quercetin and galangin may be promising therapeutic agents for AD. Additionally, their combination may be a novel therapeutic strategy for the prevention of AD.
ISSN:1107-3756
1791-244X
DOI:10.3892/ijmm.2017.3296