A molecular study on Babesia spp. in cattle and ticks in West-Azerbaijan province, Iran

Furthermore, SSU rRNA gene is highly conserved, which restricts its use between closely related species.3 With these explanations, there is a need for a single, cost-effective and technically less demanding method that could specifically and differentially detect pathogens for diagnostic and epidemi...

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Bibliographic Details
Published in:Veterinary research forum 2017-01, Vol.8 (4), p.299-306
Main Authors: Rajabi, Sepideh, Esmaeilnejad, Bijan, Tavassoli, Mousa
Format: Article
Language:English
Subjects:
Acari
Acarina
Animals
Arachnids
Arthropods
Babesia
Babesiosis
Bovidae
Cattle
Deoxyribonucleic acid
DNA
Eggs
Epidemiology
Infections
Ixodidae
Livestock
New species
Original
Oviposition
Pathogens
Polymerase chain reaction
Rhipicephalus annulatus
Rhipicephalus bursa
Rhipicephalus sanguineus
rRNA
Sheep
Theileria
Ticks
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Summary:Furthermore, SSU rRNA gene is highly conserved, which restricts its use between closely related species.3 With these explanations, there is a need for a single, cost-effective and technically less demanding method that could specifically and differentially detect pathogens for diagnostic and epidemiological assessments of bovine babesiosis in endemic regions.3 Because internal transcribed spacers (ITSs) have great variability in nucleotide and length, ITS sequences were used for discriminating different geographic isolates of piroplasmids, identifying new species and differentiating between piroplasm species and subspecies.[...]multiplex-PCR (mPCR) based on ITSs offers a significant advantage over single-species detection systems for assessment of co-infection in a large number of samples.3 Regarding the bovine babesiosis importance as a lethal infection that imposed great constraints to livestock farming and also because of the paucity of data on prevalence of babesiosis among cattle in Iran, the present study as a first molecular diagnostic technique using mPCR was employed to detect and identify Babesia spp. in cattle and ticks in West-Azerbaijan province in northwest of Iran.According to a 50.0% prevalence of babesiosis in cattle in the studied region, 5.0% absolute precision and 95.0.% confidence level, a total number of 450 cattle were sampled in the present study during favorable seasons, from early May through late September 2015.[...]the eggs and oviposition ticks were frozen at -70 °C for further use.In the present study, the prevalence of Babesia infection in cattle detected by PCR (8.9%) was significantly higher than one diagnosed through microscopic examination of thin blood smears (4.0%).[...]DNA amplification methods had higher efficiency than microscopic examination for detection of Babesia.
ISSN:2008-8140
2322-3618