Sox5 contributes to prostate cancer metastasis and is a master regulator of TGF-β-induced epithelial mesenchymal transition through controlling Twist1 expression

• Published in: British journal of cancer 2018, Vol.118 (1), p.88-97
• Main Authors: Hu, Jieping, Tian, Jing, Zhu, Shimiao, Sun, Libin, Yu, Jianpeng, Tian, Hao, Dong, Qian, Luo, Qiang, Jiang, Ning, Niu, Yuanjie, Shang, Zhiqun
• Format: Article
• Language: English
• Subjects: 692/4028/67/322; 692/4028/67/589/466; Animals; Biomedical and Life Sciences; Biomedicine; Cancer Research; Cell Line, Tumor; Disease Progression; Drug Resistance; Epidemiology; Epithelial-Mesenchymal Transition; Gene Expression Regulation, Neoplastic; Gene Knockdown Techniques; Humans; Male; Mice; Molecular Diagnostics; Molecular Medicine; Neoplasm Metastasis; Neoplasm Transplantation; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Oncology; Promoter Regions, Genetic; Prostatic Neoplasms, Castration-Resistant - genetics; Prostatic Neoplasms, Castration-Resistant - metabolism; Prostatic Neoplasms, Castration-Resistant - pathology; Signal Transduction; Smad3 Protein - metabolism; SOXD Transcription Factors - genetics; SOXD Transcription Factors - metabolism; Survival Analysis; Transforming Growth Factor beta - metabolism; Twist-Related Protein 1 - genetics; Twist-Related Protein 1 - metabolism; Up-Regulation
• ISSN: 0007-0920; 1532-1827
• DOI: 10.1038/bjc.2017.372

Background: Metastatic castration-resistant prostate cancer (mCRPC) is one of the main contributors to the death of prostate cancer patients. To date, the detailed molecular mechanisms underlying mCRPC are unclear. Given the crucial role of epithelial–mesenchymal transition (EMT) in cancer metastasis, we aimed to analyse the expression and function of Transforming growth factor-beta (TGF- β ) signal-associated protein named Sox5 in mCRPC. Methods: The protein expression levels were analysed by western blot, immunohistochemistry and immunofluorescence. Luciferase reporter assays and chromatin immunoprecipitation were employed to validate the target of Sox5. The effect of Smad3/Sox5/Twist1 on PCa progression was investigated in vitro and in vivo . Results: Here, we found that TGF- β -induced EMT was accompanied by increased Sox5 expression. Interestingly, knockdown of Sox5 expression attenuated EMT induced by TGF- β signalling. Furthermore, we demonstrated that Smad3 could bind to the promoter of Sox5 and regulate its expression. Mechanistically, Sox5 could bind to Twist1 promoter and active Twist1, which initiated EMT. Importantly, knockdown of Sox5 in prostate cancer cells resulted in less of the mesenchymal phenotype and cell migration ability. Furthermore, targeting Sox5 could inhibit prostate cancer progression in a xenograft mouse model. In clinic, patients with high Sox5 expression were more likely to suffer from metastases, and high Sox5 expression also has a lower progression-free survival and cancer specific-survival in clinic database. Conclusions: Therefore, we propose a new mechanism in which Smad3/Sox5/Twist1 promotes EMT and contributes to PCa progression.