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Cultivation of Peptidiphaga gingivicola from subgingival plaque: The first representative of a novel genus of Actinomycetaceae
Summary A novel bacterium was isolated from the subgingival plaque of a patient with periodontal disease. Bacterial strain BA112T is a facultative Gram‐positive coccus. It metabolizes alanine, arginine, glycine, histidine, leucine, proline, serine and tyrosine, but does not appear to use carbohydrat...
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| Published in: | Molecular oral microbiology 2018-02, Vol.33 (1), p.105-110 |
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| container_end_page | 110 |
| container_issue | 1 |
| container_start_page | 105 |
| container_title | Molecular oral microbiology |
| container_volume | 33 |
| creator | Beall, C.J. Mokrzan, E.M. Griffen, A.L. Leys, E.J. |
| description | Summary
A novel bacterium was isolated from the subgingival plaque of a patient with periodontal disease. Bacterial strain BA112T is a facultative Gram‐positive coccus. It metabolizes alanine, arginine, glycine, histidine, leucine, proline, serine and tyrosine, but does not appear to use carbohydrates. Urease, esculin, indole, catalase and nitrate reduction tests were all negative. Major cellular fatty acids were C18:0, C12:0, C16:0, C18:1 w9c and C20:0. The genome was sequenced and is 2.4 Mbp in length and has 64% GC content. Based on phylogenetics of the 16S rRNA sequence and concatenated alignments of 37 conserved proteins, BA112T belongs to the family Actinomycetaceae but is located on a branch of the tree without currently named members. Based on our phenotypic and phylogenetic studies, we propose that BA112T is the first known representative of a new genus, for which the name Peptidiphaga gingivicola gen. nov., sp. nov. is proposed. The type strain is BA112T. |
| doi_str_mv | 10.1111/omi.12205 |
| format | article |
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A novel bacterium was isolated from the subgingival plaque of a patient with periodontal disease. Bacterial strain BA112T is a facultative Gram‐positive coccus. It metabolizes alanine, arginine, glycine, histidine, leucine, proline, serine and tyrosine, but does not appear to use carbohydrates. Urease, esculin, indole, catalase and nitrate reduction tests were all negative. Major cellular fatty acids were C18:0, C12:0, C16:0, C18:1 w9c and C20:0. The genome was sequenced and is 2.4 Mbp in length and has 64% GC content. Based on phylogenetics of the 16S rRNA sequence and concatenated alignments of 37 conserved proteins, BA112T belongs to the family Actinomycetaceae but is located on a branch of the tree without currently named members. Based on our phenotypic and phylogenetic studies, we propose that BA112T is the first known representative of a new genus, for which the name Peptidiphaga gingivicola gen. nov., sp. nov. is proposed. The type strain is BA112T.</description><identifier>ISSN: 2041-1006</identifier><identifier>EISSN: 2041-1014</identifier><identifier>DOI: 10.1111/omi.12205</identifier><identifier>PMID: 29105370</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>actinobacteria ; Actinomycetaceae ; Actinomycetaceae - classification ; Actinomycetaceae - genetics ; Actinomycetaceae - growth & development ; Actinomycetaceae - isolation & purification ; Alanine ; Arginine ; Bacteria ; Bacterial Typing Techniques ; Base Composition ; Carbohydrates ; Catalase ; Conserved sequence ; Cultivation ; Dental plaque ; Dental Plaque - microbiology ; DNA, Bacterial - genetics ; DNA, Ribosomal - genetics ; Esculin ; Fatty acids ; Fatty Acids - analysis ; genome ; Genomes ; Glycine ; Histidine ; Humans ; Indoles ; Leucine ; New genera ; New species ; Nitrate reduction ; Periodontal disease ; Periodontal Diseases - microbiology ; Periodontics ; Phenotype ; Phylogenetics ; Phylogeny ; Proline ; Proteins ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Sequence Analysis, DNA ; Serine ; Species Specificity ; Tyrosine ; Urease ; Whole Genome Sequencing</subject><ispartof>Molecular oral microbiology, 2018-02, Vol.33 (1), p.105-110</ispartof><rights>2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4435-1865614a32b8089508bd167265d8761f5343932423b5a31dee04570a2129e4223</citedby><cites>FETCH-LOGICAL-c4435-1865614a32b8089508bd167265d8761f5343932423b5a31dee04570a2129e4223</cites><orcidid>0000-0002-2198-9124</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29105370$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Beall, C.J.</creatorcontrib><creatorcontrib>Mokrzan, E.M.</creatorcontrib><creatorcontrib>Griffen, A.L.</creatorcontrib><creatorcontrib>Leys, E.J.</creatorcontrib><title>Cultivation of Peptidiphaga gingivicola from subgingival plaque: The first representative of a novel genus of Actinomycetaceae</title><title>Molecular oral microbiology</title><addtitle>Mol Oral Microbiol</addtitle><description>Summary
A novel bacterium was isolated from the subgingival plaque of a patient with periodontal disease. Bacterial strain BA112T is a facultative Gram‐positive coccus. It metabolizes alanine, arginine, glycine, histidine, leucine, proline, serine and tyrosine, but does not appear to use carbohydrates. Urease, esculin, indole, catalase and nitrate reduction tests were all negative. Major cellular fatty acids were C18:0, C12:0, C16:0, C18:1 w9c and C20:0. The genome was sequenced and is 2.4 Mbp in length and has 64% GC content. Based on phylogenetics of the 16S rRNA sequence and concatenated alignments of 37 conserved proteins, BA112T belongs to the family Actinomycetaceae but is located on a branch of the tree without currently named members. Based on our phenotypic and phylogenetic studies, we propose that BA112T is the first known representative of a new genus, for which the name Peptidiphaga gingivicola gen. nov., sp. nov. is proposed. The type strain is BA112T.</description><subject>actinobacteria</subject><subject>Actinomycetaceae</subject><subject>Actinomycetaceae - classification</subject><subject>Actinomycetaceae - genetics</subject><subject>Actinomycetaceae - growth & development</subject><subject>Actinomycetaceae - isolation & purification</subject><subject>Alanine</subject><subject>Arginine</subject><subject>Bacteria</subject><subject>Bacterial Typing Techniques</subject><subject>Base Composition</subject><subject>Carbohydrates</subject><subject>Catalase</subject><subject>Conserved sequence</subject><subject>Cultivation</subject><subject>Dental plaque</subject><subject>Dental Plaque - microbiology</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - genetics</subject><subject>Esculin</subject><subject>Fatty acids</subject><subject>Fatty Acids - analysis</subject><subject>genome</subject><subject>Genomes</subject><subject>Glycine</subject><subject>Histidine</subject><subject>Humans</subject><subject>Indoles</subject><subject>Leucine</subject><subject>New genera</subject><subject>New species</subject><subject>Nitrate reduction</subject><subject>Periodontal disease</subject><subject>Periodontal Diseases - microbiology</subject><subject>Periodontics</subject><subject>Phenotype</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Proline</subject><subject>Proteins</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Sequence Analysis, DNA</subject><subject>Serine</subject><subject>Species Specificity</subject><subject>Tyrosine</subject><subject>Urease</subject><subject>Whole Genome Sequencing</subject><issn>2041-1006</issn><issn>2041-1014</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kUtv1DAURi0EolXpgj-ALLGBxbS-fuTBAqka8ahUVBZlbTnJTcaVEwc7CZoNvx1PU0aAhDe2ro-OvquPkJfALiCdS9_bC-CcqSfklDMJG2Agnx7fLDsh5zHes3QEyDzPn5MTXgJTImen5Od2dpNdzGT9QH1Lv-I42caOO9MZ2tmhs4utvTO0Db6nca7WmXF0dOb7jO_o3Q5pa0OcaMAxYMRhSrYFDzZDB7-gox0OczwMrurJDr7f1ziZGg2-IM9a4yKeP95n5NvHD3fbz5ub20_X26ubTS2lUBsoMpWBNIJXBStKxYqqgSznmWqKPINWCSlKwSUXlTICGkQmVc4MB16i5Fyckferd5yrHps6hQzG6THY3oS99sbqv38Gu9OdX7TKcyilSoI3j4Lg09px0r2NNTpnBvRz1FBmwEQBGUvo63_Qez-HIa2XqBSXifIh0duVqoOPMWB7DANMH4rVqVj9UGxiX_2Z_kj-rjEBlyvwwzrc_9-kb79cr8pfc0CtCg</recordid><startdate>201802</startdate><enddate>201802</enddate><creator>Beall, C.J.</creator><creator>Mokrzan, E.M.</creator><creator>Griffen, A.L.</creator><creator>Leys, E.J.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-2198-9124</orcidid></search><sort><creationdate>201802</creationdate><title>Cultivation of Peptidiphaga gingivicola from subgingival plaque: The first representative of a novel genus of Actinomycetaceae</title><author>Beall, C.J. ; Mokrzan, E.M. ; Griffen, A.L. ; Leys, E.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4435-1865614a32b8089508bd167265d8761f5343932423b5a31dee04570a2129e4223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>actinobacteria</topic><topic>Actinomycetaceae</topic><topic>Actinomycetaceae - classification</topic><topic>Actinomycetaceae - genetics</topic><topic>Actinomycetaceae - growth & development</topic><topic>Actinomycetaceae - isolation & purification</topic><topic>Alanine</topic><topic>Arginine</topic><topic>Bacteria</topic><topic>Bacterial Typing Techniques</topic><topic>Base Composition</topic><topic>Carbohydrates</topic><topic>Catalase</topic><topic>Conserved sequence</topic><topic>Cultivation</topic><topic>Dental plaque</topic><topic>Dental Plaque - microbiology</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - genetics</topic><topic>Esculin</topic><topic>Fatty acids</topic><topic>Fatty Acids - analysis</topic><topic>genome</topic><topic>Genomes</topic><topic>Glycine</topic><topic>Histidine</topic><topic>Humans</topic><topic>Indoles</topic><topic>Leucine</topic><topic>New genera</topic><topic>New species</topic><topic>Nitrate reduction</topic><topic>Periodontal disease</topic><topic>Periodontal Diseases - microbiology</topic><topic>Periodontics</topic><topic>Phenotype</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Proline</topic><topic>Proteins</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><topic>Sequence Analysis, DNA</topic><topic>Serine</topic><topic>Species Specificity</topic><topic>Tyrosine</topic><topic>Urease</topic><topic>Whole Genome Sequencing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Beall, C.J.</creatorcontrib><creatorcontrib>Mokrzan, E.M.</creatorcontrib><creatorcontrib>Griffen, A.L.</creatorcontrib><creatorcontrib>Leys, E.J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular oral microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Beall, C.J.</au><au>Mokrzan, E.M.</au><au>Griffen, A.L.</au><au>Leys, E.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cultivation of Peptidiphaga gingivicola from subgingival plaque: The first representative of a novel genus of Actinomycetaceae</atitle><jtitle>Molecular oral microbiology</jtitle><addtitle>Mol Oral Microbiol</addtitle><date>2018-02</date><risdate>2018</risdate><volume>33</volume><issue>1</issue><spage>105</spage><epage>110</epage><pages>105-110</pages><issn>2041-1006</issn><eissn>2041-1014</eissn><abstract>Summary
A novel bacterium was isolated from the subgingival plaque of a patient with periodontal disease. Bacterial strain BA112T is a facultative Gram‐positive coccus. It metabolizes alanine, arginine, glycine, histidine, leucine, proline, serine and tyrosine, but does not appear to use carbohydrates. Urease, esculin, indole, catalase and nitrate reduction tests were all negative. Major cellular fatty acids were C18:0, C12:0, C16:0, C18:1 w9c and C20:0. The genome was sequenced and is 2.4 Mbp in length and has 64% GC content. Based on phylogenetics of the 16S rRNA sequence and concatenated alignments of 37 conserved proteins, BA112T belongs to the family Actinomycetaceae but is located on a branch of the tree without currently named members. Based on our phenotypic and phylogenetic studies, we propose that BA112T is the first known representative of a new genus, for which the name Peptidiphaga gingivicola gen. nov., sp. nov. is proposed. The type strain is BA112T.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>29105370</pmid><doi>10.1111/omi.12205</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-2198-9124</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular oral microbiology, 2018-02, Vol.33 (1), p.105-110 |
| issn | 2041-1006 2041-1014 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5771945 |
| source | Wiley |
| subjects | actinobacteria Actinomycetaceae Actinomycetaceae - classification Actinomycetaceae - genetics Actinomycetaceae - growth & development Actinomycetaceae - isolation & purification Alanine Arginine Bacteria Bacterial Typing Techniques Base Composition Carbohydrates Catalase Conserved sequence Cultivation Dental plaque Dental Plaque - microbiology DNA, Bacterial - genetics DNA, Ribosomal - genetics Esculin Fatty acids Fatty Acids - analysis genome Genomes Glycine Histidine Humans Indoles Leucine New genera New species Nitrate reduction Periodontal disease Periodontal Diseases - microbiology Periodontics Phenotype Phylogenetics Phylogeny Proline Proteins RNA, Ribosomal, 16S - genetics rRNA 16S Sequence Analysis, DNA Serine Species Specificity Tyrosine Urease Whole Genome Sequencing |
| title | Cultivation of Peptidiphaga gingivicola from subgingival plaque: The first representative of a novel genus of Actinomycetaceae |
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