NGS Reveals Molecular Pathways Affected by Obesity and Weight Loss-Related Changes in miRNA Levels in Adipose Tissue

Both obesity and weight loss may cause molecular changes in adipose tissue. This study aimed to characterize changes in adipose tissue miRNome in order to identify molecular pathways affected by obesity and weight changes. Next generation sequencing (NGS) was applied to identify microRNAs (miRNAs) d...

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Bibliographic Details
Published in:International journal of molecular sciences 2017-12, Vol.19 (1), p.66
Main Authors: Kuryłowicz, Alina, Wicik, Zofia, Owczarz, Magdalena, Jonas, Marta Izabela, Kotlarek, Marta, Świerniak, Michał, Lisik, Wojciech, Jonas, Maurycy, Noszczyk, Bartłomiej, Puzianowska-Kuźnicka, Monika
Format: Article
Language:English
Subjects:
Adipose tissue
Bioinformatics
Body weight loss
miRNA
Obesity
Weight control
Weight loss
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Summary:Both obesity and weight loss may cause molecular changes in adipose tissue. This study aimed to characterize changes in adipose tissue miRNome in order to identify molecular pathways affected by obesity and weight changes. Next generation sequencing (NGS) was applied to identify microRNAs (miRNAs) differentially expressed in 47 samples of visceral (VAT) and subcutaneous (SAT) adipose tissues from normal-weight (N), obese (O) and obese after surgery-induced weight loss (PO) individuals. Subsequently miRNA expression was validated by real-time PCR in 197 adipose tissues and bioinformatics analysis performed to identify molecular pathways affected by obesity-related changes in miRNA expression. NGS identified 344 miRNAs expressed in adipose tissues with ≥5 reads per million. Using >2 and
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms19010066