Novel Method Reveals a Narrow Phylogenetic Distribution of Bacterial Dispersers in Environmental Communities Exposed to Low-Hydration Conditions

• Published in: Applied and environmental microbiology 2018-04, Vol.84 (7), p.e02857-17
• Main Authors: Krüger, U S, Bak, F, Aamand, J, Nybroe, O, Badawi, N, Smets, B F, Dechesne, A
• Format: Article
• Language: English
• Subjects: Bacteria; Bacterial infections; Bacteriological Techniques - methods; Communities; Dispersal; Dispersion; Gene sequencing; Hydration; Lakes; Lakes - microbiology; Methods; Microbiota; Microorganisms; Models, Biological; Mutants; Phylogenetics; Phylogeny; Porous materials; Pseudomonas putida; RNA, Bacterial - analysis; RNA, Ribosomal, 16S - analysis; rRNA 16S; Sequence Analysis, RNA; Soil Microbiology; Soil microorganisms; Soil water; Soils; Species; Spotlight; Strains (organisms); Studies; Surface Properties
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.02857-17

In this study, we developed a method that provides profiles of community-level surface dispersal from environmental samples under controlled hydration conditions and enables us to isolate and uncover the diversity of the fastest bacterial dispersers. The method expands on the porous surface model (PSM), previously used to monitor the dispersal of individual bacterial strains in liquid films at the surface of a porous ceramic disc. The novel procedure targets complex communities and captures the dispersed bacteria on a solid medium for growth and detection. The method was first validated by distinguishing motile and strains from their nonmotile mutants. Applying the method to soil and lake water bacterial communities showed that community-scale dispersal declined as conditions became drier. However, for both communities, dispersal was detected even under low-hydration conditions (matric potential, -3.1 kPa) previously proven too dry for strain KT2440 motility. We were then able to specifically recover and characterize the fastest dispersers from the inoculated communities. For both soil and lake samples, 16S rRNA gene amplicon sequencing revealed that the fastest dispersers were substantially less diverse than the total communities. The dispersing fraction of the soil microbial community was dominated by species cells, which increased in abundance under low-hydration conditions, while the dispersing fraction of the lake community was dominated by species cells and, under wet conditions (-0.5 kPa), also by species cells. The results gained in this study bring us a step closer to assessing the dispersal ability within complex communities under environmentally relevant conditions. Dispersal is a key process of bacterial community assembly, and yet, very few attempts have been made to assess bacterial dispersal at the community level, as the focus has previously been on pure-culture studies. A crucial factor for dispersal in habitats where hydration conditions vary, such as soils, is the thickness of the liquid films surrounding solid surfaces, but little is known about how the ability to disperse in such films varies within bacterial communities. Therefore, we developed a method to profile community dispersal and identify fast dispersers on a rough surface resembling soil surfaces. Our results suggest that within the motile fraction of a bacterial community, only a minority of the bacterial types are able to disperse in the thinnest liquid films. During dry pe