Revealing the specificity of regulatory T cells in murine autoimmune diabetes

• Published in: Proceedings of the National Academy of Sciences - PNAS 2018-05, Vol.115 (20), p.5265-5270
• Main Authors: Spence, Allyson, Purtha, Whitney, Tam, Janice, Dong, Shen, Kim, Youmin, Ju, Chia-Hsin, Sterling, Teague, Nakayama, Maki, Robinson, William H., Bluestone, Jeffrey A., Anderson, Mark S., Tang, Qizhi
• Format: Article
• Language: English
• Subjects: Animals; Antigens; Autoantigens - immunology; Autoimmune diseases; Autoimmune Diseases - immunology; Autoimmune Diseases - therapy; Autoimmunity; Biological Sciences; CD103 antigen; CD28 antigen; Cell activation; Diabetes; Diabetes mellitus; Diabetes Mellitus, Type 1 - immunology; Diabetes Mellitus, Type 1 - therapy; Disease Models, Animal; Homeostasis; Immune Tolerance - immunology; Immunoregulation; Inflammation; Insulin; Insulin - immunology; Islets of Langerhans Transplantation - immunology; Lymph nodes; Lymphocytes; Lymphocytes T; Mice; Mice, Inbred NOD; Mice, SCID; Pathogenesis; Spleen; T cell receptors; T-Lymphocytes, Regulatory - immunology
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.1715590115

Regulatory T cells (Tregs) control organ-specific autoimmunity in a tissue antigen-specific manner, yet little is known about their specificity in a natural repertoire. In this study, we used the nonobese diabetic (NOD) mouse model of autoimmune diabetes to investigate the antigen specificity of Tregs present in the inflamed tissue, the islets of Langerhans. Compared with Tregs present in spleen and lymph node, Tregs in the islets showed evidence of antigen stimulation that correlated with higher proliferation and expression of activation markers CD103, ICOS, and TIGIT. T cell receptor (TCR) repertoire profiling demonstrated that islet Treg clonotypes are expanded in the islets, suggesting localized antigen-driven expansion in inflamed islets. To determine their specificity, we captured TCRαβ pairs from islet Tregs using single-cell TCR sequencing and found direct evidence that some of these TCRs were specific for islet-derived antigens including insulin B:9–23 and proinsulin. Consistently, insulin B:9–23 tetramers readily detected insulin-specific Tregs in the islets of NOD mice. Lastly, islet Tregs from prediabetic NOD mice were effective at preventing diabetes in Treg-deficient NOD.CD28−/− recipients. These results provide a glimpse into the specificities of Tregs in a natural repertoire that are crucial for opposing the progression of autoimmune diabetes.