Stability and reproducibility of proteomic profiles measured with an aptamer-based platform

The feasibility of SOMAscan, a multiplex, high sensitivity proteomics platform, for use in studies using archived plasma samples has not yet been assessed. We quantified 1,305 proteins from plasma samples donated by 16 Nurses’ Health Study (NHS) participants, 40 NHSII participants, and 12 local volu...

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Bibliographic Details
Published in:Scientific reports 2018-05, Vol.8 (1), p.8382-10, Article 8382
Main Authors: Kim, Claire H., Tworoger, Shelley S., Stampfer, Meir J., Dillon, Simon T., Gu, Xuesong, Sawyer, Sherilyn J., Chan, Andrew T., Libermann, Towia A., Eliassen, A. Heather
Format: Article
Language:English
Subjects:
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82/80
Adult
Aging - metabolism
Aptamers
Aptamers, Nucleotide - metabolism
Body Mass Index
Correlation coefficient
Edetic acid
Fasting
Female
Heparin
Hormones
Humanities and Social Sciences
Humans
Male
multidisciplinary
Proteins
Proteomics
Proteomics - methods
Reproducibility
Reproducibility of Results
Science
Science (multidisciplinary)
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Summary:The feasibility of SOMAscan, a multiplex, high sensitivity proteomics platform, for use in studies using archived plasma samples has not yet been assessed. We quantified 1,305 proteins from plasma samples donated by 16 Nurses’ Health Study (NHS) participants, 40 NHSII participants, and 12 local volunteers. We assessed assay reproducibility using coefficients of variation (CV) from duplicate samples and intra-class correlation coefficients (ICC) and Spearman correlation coefficients (r) of samples processed (i.e., centrifuged and aliquoted into separate components) immediately, 24, and 48 hours after collection, as well as those of samples collected from the same individuals 1 year apart. CVs were
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-26640-w