Effective BAC clone anchoring with genotyping-by-sequencing and Diversity Arrays Technology in a large genome cereal rye

Identification of bacterial artificial chromosome (BAC) clones containing specific sequences is a prerequisite for many applications, such as physical map anchoring or gene cloning. Existing BAC library screening strategies are either low-throughput or require a considerable initial input of resourc...

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Bibliographic Details
Published in:Scientific reports 2018-05, Vol.8 (1), p.8428-9, Article 8428
Main Authors: Borzęcka, Ewa, Hawliczek-Strulak, Anna, Bolibok, Leszek, Gawroński, Piotr, Tofil, Katarzyna, Milczarski, Paweł, Stojałowski, Stefan, Myśków, Beata, Targońska-Karasek, Małgorzata, Grądzielewska, Agnieszka, Smolik, Miłosz, Kilian, Andrzej, Bolibok-Brągoszewska, Hanna
Format: Article
Language:English
Subjects:
45/22
45/23
45/47
45/61
631/208/721
631/208/726
631/449
Artificial chromosomes
Bacterial artificial chromosomes
Cloning
DNA microarrays
Genetic markers
Genomes
Genotyping
Humanities and Social Sciences
multidisciplinary
Science
Science (multidisciplinary)
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Summary:Identification of bacterial artificial chromosome (BAC) clones containing specific sequences is a prerequisite for many applications, such as physical map anchoring or gene cloning. Existing BAC library screening strategies are either low-throughput or require a considerable initial input of resources for platform establishment. We describe a high-throughput, reliable, and cost-effective BAC library screening approach deploying genotyping platforms which are independent from the availability of sequence information: a genotyping-by-sequencing (GBS) method DArTSeq and the microarray-based Diversity Arrays Technology (DArT). The performance of these methods was tested in a very large and complex rye genome. The DArTseq approach delivered superior results: a several fold higher efficiency of addressing genetic markers to BAC clones and anchoring of BAC clones to genetic map and also a higher reliability. Considering the sequence independence of the platform, the DArTseq-based library screening can be proposed as an attractive method to speed up genomics research in resource poor species.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-26541-y