In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration

Purpose The study aims to test the hypothesis that platelet-rich plasma (PRP) stimulates cellular processes involved in endometrial regeneration relevant to clinical management of poor endometrial growth or intrauterine scarring. Methods Human endometrial stromal fibroblasts (eSF), endometrial mesen...

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Bibliographic Details
Published in:Journal of assisted reproduction and genetics 2018-05, Vol.35 (5), p.757-770
Main Authors: Aghajanova, Lusine, Houshdaran, Sahar, Balayan, Shaina, Manvelyan, Evelina, Irwin, Juan C., Huddleston, Heather G., Giudice, Linda C.
Format: Article
Language:English
Subjects:
Adenocarcinoma
Aged
Autografts
Bone marrow
Cell growth
Cell migration
Cell Movement
Cell Proliferation
Cells, Cultured
Chemokines
Chemotaxis
Cytokeratin
Endometrium
Endometrium - cytology
Endometrium - physiology
Epithelial-Mesenchymal Transition
Estrogens
Female
Fibroblasts
Fibroblasts - physiology
Gene expression
Gene Expression Regulation
Gynecology
Human Genetics
Humans
Male
Matrilysin
Medicine
Medicine & Public Health
Mesenchymal Stem Cells - physiology
Mesenchyme
Platelet-Rich Plasma
Platelets
Progesterone
Regeneration
Reproductive Medicine
Reproductive Physiology and Disease
Stem cells
Vimentin
Wound healing
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Summary:Purpose The study aims to test the hypothesis that platelet-rich plasma (PRP) stimulates cellular processes involved in endometrial regeneration relevant to clinical management of poor endometrial growth or intrauterine scarring. Methods Human endometrial stromal fibroblasts (eSF), endometrial mesenchymal stem cells (eMSC), bone marrow-derived mesenchymal stem cells (BM-MSC), and Ishikawa endometrial adenocarcinoma cells (IC) were cultured with/without 5% activated (a) PRP, non-activated (na) PRP, aPPP (platelet-poor-plasma), and naPPP. Treatment effects were evaluated with cell proliferation (WST-1), wound healing, and chemotaxis Transwell migration assays. Mesenchymal-to-epithelial transition (MET) was evaluated by cytokeratin and vimentin expression. Differential gene expression of various markers was analyzed by multiplex Q-PCR. Results Activated PRP enhanced migration of all cell types, compared to naPRP, aPPP, naPPP, and vehicle controls, in a time-dependent manner ( p  
ISSN:1058-0468
1573-7330
DOI:10.1007/s10815-018-1130-8