Genotyping comparison of Mycobacterium leprae isolates by VNTR analysis from nasal samples in a Brazilian endemic region

This study analyzed the genetic diversity by MIRU-VNTR of Mycobacterium leprae isolates from nasal cavities and related to epidemiological and clinical data. The sample consisted of 48 newly diagnosed leprosy cases that tested positive for M. leprae PCR in nasal secretion (NS) attending to the Natio...

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Bibliographic Details
Published in:Pathogens and global health 2018-03, Vol.112 (2), p.79-85
Main Authors: Lima, Luana Nepomueceno Costa, Frota, Cristiane Cunha, Suffys, Phillip Noel, Fontes, Amanda Nogueira Brum, Mota, Rosa Maria Salani, Almeida, Rosa Livia Freitas, Andrade Pontes, Maria Araci de, Gonçalves, Heitor de Sá, Kendall, Carl, Kerr, Ligia Regina Sansigolo
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Body Fluids - microbiology
Brazil
Child
Cluster Analysis
Cross-Sectional Studies
Female
Genetic Variation
Genotype
Genotyping Techniques - methods
Humans
Leprosy - microbiology
Male
Middle Aged
Minisatellite Repeats
Mycobacterium leprae - classification
Mycobacterium leprae - genetics
Mycobacterium leprae - isolation & purification
Nasal Cavity - microbiology
Original
Young Adult
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Summary:This study analyzed the genetic diversity by MIRU-VNTR of Mycobacterium leprae isolates from nasal cavities and related to epidemiological and clinical data. The sample consisted of 48 newly diagnosed leprosy cases that tested positive for M. leprae PCR in nasal secretion (NS) attending to the National Reference Center of Dermatology Dona Libania (CDERM), Fortaleza, Brazil. Total DNA was extracted from NS of each patient and used for amplification of four M. leprae VNTR loci. Four clusters of M. leprae isolates were formed with identical genotypes. In the spatial analysis, 12 leprosy cases presented similar genotypes organized into 4 clusters. The most common genotypes in the current study was AC8b: 8, AC9: 7, AC8a: 8, GTA9: 10, which may represent a genotype of circulating strains most often in Ceará. A minimum set of four MIRU-VNTR loci was demonstrated to study the genetic diversity of M. leprae isolates from NS.
ISSN:2047-7724
2047-7732
DOI:10.1080/20477724.2018.1427308