Detachment of ligands from nanoparticle surface under flow and endothelial cell contact: Assessment using microfluidic devices

Surface modification of nanoparticles is a well‐established methodology to alter their properties to enhance circulation half‐life. While literature studies using conventional, in vitro characterization are routinely used to evaluate the biocompatibility of such modifications, relatively little atte...

Full description

Saved in:
Bibliographic Details
Published in:Bioengineering & translational medicine 2018-05, Vol.3 (2), p.148-155
Main Authors: Jarvis, Maria, Arnold, Michael, Ott, Jenna, Krishnan, Vinu, Pant, Kapil, Prabhakarpandian, Balabhaskar, Mitragotri, Samir
Format: Article
Language:English
Subjects:
Acids
Biocompatibility
degradation
Endothelial cells
Folic acid
Laboratories
Ligands
Lipids
microfluidic device
Microfluidic devices
microvascular
Nanocrystals
Nanoparticles
Parameter modification
Polyethylene glycol
Research Report
Research Reports
shear
Stability analysis
Studies
Surface charge
Surface stability
targeting
translation
Vitamin B
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Surface modification of nanoparticles is a well‐established methodology to alter their properties to enhance circulation half‐life. While literature studies using conventional, in vitro characterization are routinely used to evaluate the biocompatibility of such modifications, relatively little attention has been paid to assess the stability of such surface modifications in physiologically relevant conditions. Here, microfluidic devices were used to study the effect of factors that adversely impact surface modifications including vascular flow and endothelial cell interactions. Camptothecin nanoparticles coated with polyethylene glycol (PEG) and/or folic acid were analyzed using linear channels and microvascular networks. Detachment of PEG was observed in cell‐free conditions and was attributed to interplay between the flow and method of PEG attachment. The flow and cells also impacted the surface charge of nanoparticles. Presence of endothelial cells further increased PEG shedding. The results demonstrate that endothelial cell contact, and vascular flow parameters modify surface ligands on nanoparticle surfaces. The manuscript provides a methodology to assess the detachment of ligands from the nanoparticle surface when exposed to flow and contact with endothelial cells.
ISSN:2380-6761
2380-6761
DOI:10.1002/btm2.10089