Pluripotent stem cells as a source of osteoblasts for bone tissue regeneration

Appropriate and abundant sources of bone-forming osteoblasts are essential for bone tissue engineering. Pluripotent stem cells can self-renew and thereby offer a potentially unlimited supply of osteoblasts, a significant advantage over other cell sources. We generated mouse embryonic stem cells (ESC...

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Bibliographic Details
Published in:Biomaterials 2019-03, Vol.196, p.31-45
Main Authors: Zhu, Hui, Kimura, Takaharu, Swami, Srilatha, Wu, Joy Y.
Format: Article
Language:English
Subjects:
Animals
Bone Regeneration - genetics
Bone Regeneration - physiology
Cell Differentiation - genetics
Cell Line
Differentiation
Embryonic stem cells
Green Fluorescent Proteins - metabolism
Induced pluripotent stem cells
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Mice
Mouse Embryonic Stem Cells - cytology
Osteoblasts
Osteoblasts - cytology
Osteoblasts - metabolism
Pluripotent Stem Cells - cytology
Skull - cytology
Tissue engineering
Transcriptome - genetics
Up-Regulation - genetics
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Summary:Appropriate and abundant sources of bone-forming osteoblasts are essential for bone tissue engineering. Pluripotent stem cells can self-renew and thereby offer a potentially unlimited supply of osteoblasts, a significant advantage over other cell sources. We generated mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) from transgenic mice expressing rat 2.3 kb type I collagen promoter-driven green fluorescent protein (Col2.3GFP), a reporter of the osteoblast lineage. We demonstrated that Col2.3GFP ESCs and iPSCs can be successfully differentiated to osteoblast lineage cells that express Col2.3GFP in vitro. We harvested GFP+ osteoblasts differentiated from ESCs. Genome wide gene expression profiles validated that ESC- and iPSC-derived osteoblasts resemble calvarial osteoblasts, and that Col2.3GFP expression serves as a marker for mature osteoblasts. Our results confirm the cell identity of ESC- and iPSC-derived osteoblasts and highlight the potential of pluripotent stem cells as a source of osteoblasts for regenerative medicine.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2018.02.009