Hydrogenotrophic Methanogenesis and Autotrophic Growth of Methanosarcina thermophila

Although Methanosarcinales are versatile concerning their methanogenic substrates, the ability of Methanosarcina thermophila to use carbon dioxide (CO2) for catabolic and anabolic metabolism was not proven until now. Here, we show that M. thermophila used CO2 to perform hydrogenotrophic methanogenes...

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Bibliographic Details
Published in:Archaea (Vancouver) 2018-01, Vol.2018 (2018), p.1-7
Main Authors: Illmer, Paul, Wagner, Andreas Otto, Hintersonnleitner, Anna, Lackner, Nina
Format: Article
Language:English
Subjects:
Analysis
Autotrophic Processes
Bacteria
Bacteriology
Biomass
Carbon dioxide
Carbon Dioxide - metabolism
Cluster Analysis
Deoxyribonucleic acid
DNA
DNA, Archaeal - chemistry
DNA, Archaeal - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Gases
Gene sequencing
Genomic analysis
Growth
Hydrogen
Hydrogen - metabolism
Metabolism
Methane
Methane - metabolism
Methanogenesis
Methanol
Methanol - metabolism
Methanosarcina - classification
Methanosarcina - growth & development
Methanosarcina - isolation & purification
Methanosarcina - metabolism
Methanosarcina thermophila
Microbiology
Microorganisms
Phylogeny
Physiological aspects
Physiology
Ribonucleic acid
RNA
RNA sequencing
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Substrates
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Summary:Although Methanosarcinales are versatile concerning their methanogenic substrates, the ability of Methanosarcina thermophila to use carbon dioxide (CO2) for catabolic and anabolic metabolism was not proven until now. Here, we show that M. thermophila used CO2 to perform hydrogenotrophic methanogenesis in the presence as well as in the absence of methanol. During incubation with hydrogen, the methanogen utilized the substrates methanol and CO2 consecutively, resulting in a biphasic methane production. Growth exclusively from CO2 occurred slowly but reproducibly with concomitant production of biomass, verified by DNA quantification. Besides verification through multiple transfers into fresh medium, the identity of the culture was confirmed by 16s RNA sequencing, and the incorporation of carbon atoms from 13CO2 into 13CH4 molecules was measured to validate the obtained data. New insights into the physiology of M. thermophila can serve as reference for genomic analyses to link genes with metabolic features in uncultured organisms.
ISSN:1472-3646
1472-3654
DOI:10.1155/2018/4712608