Untargeted metabolomics analysis of ischemia–reperfusion-injured hearts ex vivo from sedentary and exercise-trained rats

Introduction The effects of exercise on the heart and its resistance to disease are well-documented. Recent studies have identified that exercise-induced resistance to arrhythmia is due to the preservation of mitochondrial membrane potential. Objectives To identify novel metabolic changes that occur...

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Bibliographic Details
Published in:Metabolomics 2018-01, Vol.14 (1), p.1-15, Article 8
Main Authors: Parry, Traci L., Starnes, Joseph W., O’Neal, Sara K., Bain, James R., Muehlbauer, Michael J., Honcoop, Aubree, Ilaiwy, Amro, Christopher, Peter, Patterson, Cam, Willis, Monte S.
Format: Article
Language:English
Subjects:
Ammonia
Antioxidants
Arrhythmia
Biochemistry
Biomedical and Life Sciences
Biomedicine
Biosynthesis
Cell Biology
Developmental Biology
Disease resistance
Enrichment
Heart diseases
Injury analysis
Ischemia
Life Sciences
Membrane potential
Metabolism
Metabolites
Metabolomics
Mitochondria
Molecular Medicine
Oleic acid
Original Article
Oxygen consumption
Pantothenic acid
Preservation
Protein biosynthesis
Protein folding
Protein synthesis
Proteins
Quality control
Rats
Reperfusion
Sedentary behavior
Statistical analysis
Variance analysis
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Summary:Introduction The effects of exercise on the heart and its resistance to disease are well-documented. Recent studies have identified that exercise-induced resistance to arrhythmia is due to the preservation of mitochondrial membrane potential. Objectives To identify novel metabolic changes that occur parallel to these mitochondrial alterations, we performed non-targeted metabolomics analysis on hearts from sedentary and exercise-trained rats challenged with isolated heart ischemia–reperfusion injury (I/R). Methods Eight-week old Sprague–Dawley rats were treadmill trained 5 days/week for 6 weeks (exercise duration and intensity progressively increased to 1 h at 30 m/min up a 10.5% incline, 75–80% VO 2max ). The recovery of pre-ischemic function for sedentary rat hearts was 28.8 ± 5.4% (N = 12) compared to exercise trained hearts, which recovered 51.9% ± 5.7 (N = 14) (p 
ISSN:1573-3882
1573-3890
DOI:10.1007/s11306-017-1303-y