Natural antimicrobials for beet leaves preservation: in vitro and in vivo determination of effectiveness

Nisin (Ni), natamycin (Na), green tea extract (GTE) and their combinations were evaluated for controlling beet leaves’ native microbiota as well as Listeria innocua and Escherichia coli external contaminations. Antimicrobial effectiveness was evaluated through in vitro and in vivo studies. In the in...

Full description

Saved in:
Bibliographic Details
Published in:Journal of food science and technology 2018-09, Vol.55 (9), p.3665-3674
Main Authors: Fernandez, María Verónica, Jagus, Rosa Juana, Agüero, María Victoria
Format: Article
Language:English
Subjects:
Aerobic bacteria
Antiinfectives and antibacterials
Antimicrobial activity
Antimicrobial agents
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
E coli
Food Science
Green tea
In vivo methods and tests
Leaves
Listeria
Listeria innocua
Microbiota
Microorganisms
Natamycin
Nisin
Nutrition
Original
Original Article
Pasteurization
Preservation
Spoilage
Studies
Tea
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nisin (Ni), natamycin (Na), green tea extract (GTE) and their combinations were evaluated for controlling beet leaves’ native microbiota as well as Listeria innocua and Escherichia coli external contaminations. Antimicrobial effectiveness was evaluated through in vitro and in vivo studies. In the in vitro studies, GTE treatment (0.85%) completely eliminated growth of native microbiota, reduced L. innocua from values of 8.5–3.5 log from 24 h onwards and reduced E. coli below detection limit (DL) after 72 h. Ni (500 IU/mL) was the most effective against L. innocua (7 log CFU/mL reduction) and its combination with GTE presented significant interactions for mesophilic aerobic bacteria (MAB) and L. innocua control. Na (200 ppm) alone or in combination with GTE did not show antimicrobial activity against microorganisms under study. Additionally in vivo evaluation showed that 2.5–5% GTE concentrations are needed to achieve significant inhibitory effects on MAB, L. innocua and E. coli . Furthermore, the best results for MAB and L. innocua control were obtained with the GTE5 + Ni treatment. This study revealed that GTE, either alone or combined with nisin, is a highly promising option with potential for reducing or preventing the growth of pathogenic and spoilage microorganisms present in leafy vegetables, specifically in beet leaves.
ISSN:0022-1155
0975-8402
DOI:10.1007/s13197-018-3295-7