Mastering Complexity: Towards Bottom-up Construction of Multifunctional Eukaryotic Synthetic Cells

• Published in: Trends in biotechnology (Regular ed.) 2018-09, Vol.36 (9), p.938-951
• Main Authors: Göpfrich, Kerstin, Platzman, Ilia, Spatz, Joachim P.
• Format: Article
• Language: English
• Subjects: Artificial Cells - chemistry; Artificial Cells - cytology; Biological Transport; Biomimetic Materials - chemistry; Biomimetic Materials - metabolism; bottom-up synthetic biology; Cell Compartmentation; Cell Engineering - instrumentation; Cell Engineering - methods; Cell Membrane - chemistry; Cell Membrane - metabolism; compartment; Complexity; Cytoskeleton - chemistry; Cytoskeleton - ultrastructure; Deoxyribonucleic acid; DNA; DNA - chemistry; DNA - metabolism; DNA nanotechnology; droplet-based microfluidics; Eukaryotes; Eukaryotic Cells - cytology; Eukaryotic Cells - physiology; Gene expression; giant unilamellar vesicle; Humans; Intracellular Membranes - chemistry; Intracellular Membranes - metabolism; Lipids; Mastering; Microfluidics; Microfluidics - instrumentation; Microfluidics - methods; Mimicry; Modules; Nanotechnology; Nanotechnology - instrumentation; Nanotechnology - methods; Organelles; Organelles - chemistry; Organelles - metabolism; Origin of Life; Synthetic biology; Synthetic Biology - instrumentation; Synthetic Biology - methods; synthetic cell; Unilamellar Liposomes - chemistry; Unilamellar Liposomes - metabolism
• ISSN: 0167-7799; 1879-3096
• DOI: 10.1016/j.tibtech.2018.03.008

With the ultimate aim to construct a living cell, bottom-up synthetic biology strives to reconstitute cellular phenomena in vitro – disentangled from the complex environment of a cell. Recent work towards this ambitious goal has provided new insights into the mechanisms governing life. With the fast-growing library of functional modules for synthetic cells, their classification and integration become increasingly important. We discuss strategies to reverse-engineer and recombine functional parts for synthetic eukaryotes, mimicking the characteristics of nature’s own prototype. Particularly, we focus on large outer compartments, complex endomembrane systems with organelles, and versatile cytoskeletons as hallmarks of eukaryotic life. Moreover, we identify microfluidics and DNA nanotechnology as two technologies that can integrate these functional modules into sophisticated multifunctional synthetic cells. Bottom-up synthetic biology thrives in reverse-engineering a particular biological function using a minimal set of molecular components, like purified proteins. Recently, precision technologies, like microfluidics, have been used to recombine functional modules towards multifunctional synthetic cells. Synthetic biology can capitalize on a variety of pre-existing on-chip functions, which greatly increases the scope for complexity in the field. Advances in DNA nanotechnology gave rise to a diverse range of fully synthetic functional modules, like DNA-based ion channels or motors, which can replace some protein-based parts. Noteworthy progress has been made in achieving large and stable compartments, organelle-like multicompartment systems, and sophisticated cytoskeletal structures.