Single-neuronal cell culture and monitoring platform using a fully transparent microfluidic DEP device

Dielectrophoresis using multi-electrode arrays allows a non-invasive interface with biological cells for long-term monitoring of electrophysiological parameters as well as a label-free and non-destructive technique for neuronal cell manipulation. However, experiments for neuronal cell manipulation u...

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Bibliographic Details
Published in:Scientific reports 2018-09, Vol.8 (1), p.13194-9, Article 13194
Main Authors: Kim, Hyungsoo, Lee, In-Kyu, Taylor, Kendra, Richters, Karl, Baek, Dong-Hyun, Ryu, Jae Ha, Cho, Sang June, Jung, Yei Hwan, Park, Dong-Wook, Novello, Joseph, Bong, Jihye, Suminski, Aaron J., Dingle, Aaron M., Blick, Robert H., Williams, Justin C., Dent, Erik W., Ma, Zhenqiang
Format: Article
Language:English
Subjects:
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13/107
13/62
631/1647/1888
631/61/2035
Cell culture
Contamination
Electrodes
Humanities and Social Sciences
Microfluidics
multidisciplinary
Neural networks
Science
Science (multidisciplinary)
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Summary:Dielectrophoresis using multi-electrode arrays allows a non-invasive interface with biological cells for long-term monitoring of electrophysiological parameters as well as a label-free and non-destructive technique for neuronal cell manipulation. However, experiments for neuronal cell manipulation utilizing dielectrophoresis have been constrained because dielectrophoresis devices generally function outside of the controlled environment ( i.e . incubator) during the cell manipulation process, which is problematic because neurons are highly susceptible to the properties of the physiochemical environment. Furthermore, the conventional multi-electrode arrays designed to generate dielectrophoretic force are often fabricated with non-transparent materials that confound live-cell imaging. Here we present an advanced single-neuronal cell culture and monitoring platform using a fully transparent microfluidic dielectrophoresis device for the unabated monitoring of neuronal cell development and function. The device is mounted inside a sealed incubation chamber to ensure improved homeostatic conditions and reduced contamination risk. Consequently, we successfully trap and culture single neurons on a desired location and monitor their growth process over a week. The proposed single-neuronal cell culture and monitoring platform not only has significant potential to realize an in vitro ordered neuronal network, but also offers a useful tool for a wide range of neurological research and electrophysiological studies of neuronal networks.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-31576-2