Effect of autophagy-associated proteins on the arecoline-induced liver injury in mice

Arecoline can be used to treat diseases including glaucoma and tapeworm infection, however, long-term administration can cause severe adverse effects, including oral submucosal fibrosis, oral cancer, hepatic injury and liver cancer. Autophagy serves a role in these injuries. The present study establ...

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Bibliographic Details
Published in:Experimental and therapeutic medicine 2018-10, Vol.16 (4), p.3041-3049
Main Authors: Wang, Xia, Song, Xinhong, Si, Youjiao, Xia, Jikai, Wang, Bin, Wang, Peiyuan
Format: Article
Language:English
Subjects:
Apoptosis
Areca
Autophagy
Drug dosages
Enzymes
Eukaryotes
Gangrene
Gene expression
Humidity
Laboratory animals
Liver
Medical research
Metabolism
Metabolites
Mitochondria
Oral cancer
Phosphorylation
Physiology
Polyclonal antibodies
Proteins
Signal transduction
Studies
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Summary:Arecoline can be used to treat diseases including glaucoma and tapeworm infection, however, long-term administration can cause severe adverse effects, including oral submucosal fibrosis, oral cancer, hepatic injury and liver cancer. Autophagy serves a role in these injuries. The present study established a mouse model of arecoline-induced hepatic injury and investigated the role of autophagy-associated proteins in this injury. The results indicated that the expression levels of the autophagy marker protein microtubule associated protein 1 light chain 3 B (MAP1LC3B) and autophagy-promoting protein beclin 1 were elevated in the injured hepatic cells, while the expression levels of a well-known negative regulator of autophagy, mammalian target of rapamycin (mTOR), were reduced. Following treatment of the hepatic injury with glutathione, the liver function improved and liver damage was reduced effectively. Compared with the control group, the expression levels of both MAP1LC3B and beclin 1 were significantly upregulated in the glutathione-treated mice, but the expression of mTOR was significantly downregulated. It may be concluded that in the process of protecting against arecoline-induced hepatic injury, glutathione cooperates with mTOR and beclin 1 to accelerate autophagy, maintaining stable cell morphology and cellular functions.
ISSN:1792-0981
1792-1015
DOI:10.3892/etm.2018.6564