FISH-aimed karyotype analysis in Aconitum subgen. Aconitum reveals excessive rDNA sites in tetraploid taxa

The location of 5S and 35S rDNA sequences in chromosomes of four Aconitum subsp. Aconitum species was analyzed after fluorescence in situ hybridization (FISH). Both in diploids (2 n  = 2 x  = 16; Aconitum variegatum , A. degenii ) and tetraploids (2n = 4× = 32; A. firmum , A. plicatum ), rDNA repeat...

Full description

Saved in:
Bibliographic Details
Published in:Protoplasma 2018-09, Vol.255 (5), p.1363-1372
Main Authors: Joachimiak, Andrzej J., Hasterok, Robert, Sliwinska, Elwira, Musiał, Krystyna, Grabowska-Joachimiak, Aleksandra
Format: Article
Language:English
Subjects:
Aconitum
Biomedical and Life Sciences
Cell Biology
Chromosomes
Diploids
diploidy
Fluorescence in situ hybridization
genome
Genomes
Karyotypes
karyotyping
Life Sciences
loci
nucleotide sequences
Original
Original Article
Plant Sciences
ribosomal DNA
tetraploidy
Zoology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The location of 5S and 35S rDNA sequences in chromosomes of four Aconitum subsp. Aconitum species was analyzed after fluorescence in situ hybridization (FISH). Both in diploids (2 n  = 2 x  = 16; Aconitum variegatum , A. degenii ) and tetraploids (2n = 4× = 32; A. firmum , A. plicatum ), rDNA repeats were localized exclusively on the shorter arms of chromosomes, in subterminal or pericentromeric sites. All analyzed species showed similar basal genome size (Cx = 5.31–5.71 pg). The most striking features of tetraploid karyotypes were the conservation of diploid rDNA loci and emergence of many additional 5S rDNA clusters. Chromosomal distribution of excessive ribosomal sites suggests their role in the secondary diploidization of tetraploid karyotypes.
ISSN:0033-183X
1615-6102
DOI:10.1007/s00709-018-1238-9