tRNA diversification among uncultured archeon clones

• Published in: Bioinformation 2018-01, Vol.14 (7), p.357-360
• Main Authors: Shawan, Mohammad Mahfuz Ali Khan, Hasan, Md Ashraful, Yesmin, Raihana, Hossan, Tareq, Hossain, Md Mozammel, Hasan, Md Mahmudul, Parvin, Afroza, Morshed, Mahbubul, Salauddin, Nahiyan Mohammad, Sarker, Satya Ranjan, Rahman, Md Nazibur, Rahman, S M Badier
• Format: Article
• Language: English
• Subjects: Cloning; Cytosine; Deoxyribonucleic acid; Digital data; DNA; Gene sequencing; Genomes; Guanine; Hypothesis; Nucleotide sequence; Prokaryotes; tRNA Pro; tRNA Val
• ISSN: 0973-2063; 0973-8894; 0973-2063
• DOI: 10.6026/97320630014357

Whole genome sequences (DNA sequences) of four uncultured archeon clones (1B6:CR626858.1, 4B7:CR626856.1, 22i07:JQ768096.1 and 19c08:JQ768095.1) were collected from NCBI BioSample database for the construction of digital data on tRNA. tRNAscan-SE 2.0 and ENDMEMO tools were used to identify and sketch tRNA structure as well as calculate Guanine-Cytosine (GC) percentage respectively. Eight true/functional tRNAs were identified from above 4 sequences which showed cove score greater than 20% with no variable loop. The tRNAs from the uncultured archeon clones were classified as Ala, Arg, Ile, Thr, Pro and Val type tRNA with cove score ranging from 34.22%-79.03%. The range of GC content was found 42.89%-56.91%; while tRNA contributed GC content ranging from 52%-64.86% to the total GC content in these sequences. The data fabricated in this study could be very useful for studying the diversity of tRNA among prokaryotes.