Transcriptomics and co-expression networks reveal tissue-specific responses and regulatory hubs under mild and severe drought in papaya (Carica papaya L.)

Plants respond to drought stress through the ABA dependent and independent pathways, which in turn modulate transcriptional regulatory hubs. Here, we employed Illumina RNA-Seq to analyze a total of 18 cDNA libraries from leaves, sap, and roots of papaya plants under drought stress. Reference and de...

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Published in:Scientific reports 2018-09, Vol.8 (1), p.14539-16, Article 14539
Main Authors: Gamboa-Tuz, Samuel David, Pereira-Santana, Alejandro, Zamora-Briseño, Jesús Alejandro, Castano, Enrique, Espadas-Gil, Francisco, Ayala-Sumuano, Jorge Tonatiuh, Keb-Llanes, Miguel Ángel, Sanchez-Teyer, Felipe, Rodríguez-Zapata, Luis Carlos
Format: Article
Language:English
Subjects:
38/91
631/114/2114
631/114/2403
631/1647/48
631/449/2661/2665
631/61/514/1949
Abiotic stress
Abscisic acid
Biosynthesis
Cell cycle
DNA repair
Drought
Humanities and Social Sciences
Leaves
multidisciplinary
Oxidation
Oxidation-reduction potential
Ribonucleic acid
RNA
Roots
Science
Science (multidisciplinary)
Sucrose
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Summary:Plants respond to drought stress through the ABA dependent and independent pathways, which in turn modulate transcriptional regulatory hubs. Here, we employed Illumina RNA-Seq to analyze a total of 18 cDNA libraries from leaves, sap, and roots of papaya plants under drought stress. Reference and de novo transcriptomic analyses identified 8,549 and 6,089 drought-responsive genes and unigenes, respectively. Core sets of 6 and 34 genes were simultaneously up- or down-regulated, respectively, in all stressed samples. Moreover, GO enrichment analysis revealed that under moderate drought stress, processes related to cell cycle and DNA repair were up-regulated in leaves and sap; while responses to abiotic stress, hormone signaling, sucrose metabolism, and suberin biosynthesis were up-regulated in roots. Under severe drought stress, biological processes related to abiotic stress, hormone signaling, and oxidation-reduction were up-regulated in all tissues. Moreover, similar biological processes were commonly down-regulated in all stressed samples. Furthermore, co-expression network analysis revealed three and eight transcriptionally regulated modules in leaves and roots, respectively. Seventeen stress-related TFs were identified, potentially serving as main regulatory hubs in leaves and roots. Our findings provide insight into the molecular responses of papaya plant to drought, which could contribute to the improvement of this important tropical crop.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-32904-2