Nitric oxide responses in Arabidopsis hypocotyls are mediated by diverse phytohormone pathways

An NO-sensing mechanism controlling hypocotyl growth in etiolated seedlings requires the biosynthesis and/or signaling of ethylene, strigolactones, salicylate, abscisic acid, and brassinosteroids. Abstract Plants are often exposed to high levels of nitric oxide (NO) that affects development and stre...

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Bibliographic Details
Published in:Journal of experimental botany 2018-10, Vol.69 (21), p.5265-5278
Main Authors: Castillo, Mari-Cruz, Coego, Alberto, Costa-Broseta, Álvaro, León, José
Format: Article
Language:English
Subjects:
abscisic acid
Arabidopsis
biosynthesis
brassinosteroids
ethylene signaling pathway
etiolation
genes
genetically modified organisms
homeostasis
hormones
hypocotyls
mutants
nitric oxide
receptors
Research Papers
seedlings
strigolactones
transcription factors
transcriptome
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Summary:An NO-sensing mechanism controlling hypocotyl growth in etiolated seedlings requires the biosynthesis and/or signaling of ethylene, strigolactones, salicylate, abscisic acid, and brassinosteroids. Abstract Plants are often exposed to high levels of nitric oxide (NO) that affects development and stress-triggered responses. However, the way in which plants sense NO is still largely unknown. Here we combine the analysis of early changes in the transcriptome of plants exposed to a short acute pulse of exogenous NO with the identification of transcription factors (TFs) involved in NO sensing. The NO-responsive transcriptome was enriched in hormone homeostasis- and signaling-related genes. To assess events involved in NO sensing in hypocotyls, we used a functional sensing assay based on the NO-induced inhibition of hypocotyl elongation in etiolated seedlings. Hormone-related mutants and the TRANSPLANTA collection of transgenic lines conditionally expressing Arabidopsis TFs were screened for NO-triggered hypocotyl shortening. These approaches allowed the identification of hormone-related TFs, ethylene perception and signaling, strigolactone biosynthesis and signaling, and salicylate production and accumulation that are essential for or modulate hypocotyl NO sensing. Moreover, NO inhibits hypocotyl elongation through the positive and negative regulation of some abscisic acid (ABA) receptors and transcripts encoding brassinosteroid signaling components thereby also implicating these hormones in NO sensing.
ISSN:0022-0957
1460-2431
1460-2431
DOI:10.1093/jxb/ery286