Probing molecular pathways for DNA orientational trapping, unzipping and translocation in nanopores by using a tunable overhang sensor

Nanopores provide a unique single-molecule platform for genetic and epigenetic detection. The target nucleic acids can be accurately analyzed by characterizing their specific electric fingerprints or signatures in the nanopore. Here we report a series of novel nanopore signatures generated by target...

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Bibliographic Details
Published in:Nanoscale 2014-10, Vol.6 (19), p.11372-11379
Main Authors: Wang, Yong, Tian, Kai, Hunter, Lehr L, Ritzo, Brandon, Gu, Li-Qun
Format: Article
Language:English
Subjects:
Computer Simulation
Computer-Aided Design
DNA Probes - chemistry
DNA Probes - genetics
DNA Probes - ultrastructure
Equipment Design
Equipment Failure Analysis
Materials Testing
Models, Chemical
Models, Molecular
Motion
Nucleic Acid Conformation
Oligonucleotide Array Sequence Analysis - instrumentation
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Summary:Nanopores provide a unique single-molecule platform for genetic and epigenetic detection. The target nucleic acids can be accurately analyzed by characterizing their specific electric fingerprints or signatures in the nanopore. Here we report a series of novel nanopore signatures generated by target nucleic acids that are hybridized with a probe. A length-tunable overhang appended to the probe functions as a sensor to specifically modulate the nanopore current profile. The resulting signatures can reveal multiple mechanisms for the orientational trapping, unzipping, escaping and translocation of nucleic acids in the nanopore. This universal approach can be used to program various molecular movement pathways, elucidate their kinetics, and enhance the sensitivity and specificity of the nanopore sensor for nucleic acid detection.
ISSN:2040-3364
2040-3372
DOI:10.1039/c4nr03195d