Distinct Signatures of Host Defense Suppression by Plant-Feeding Mites

• Published in: International journal of molecular sciences 2018-10, Vol.19 (10), p.3265
• Main Authors: Schimmel, Bernardus C J, Alba, Juan M, Wybouw, Nicky, Glas, Joris J, Meijer, Tomas T, Schuurink, Robert C, Kant, Merijn R
• Format: Article
• Language: English
• Subjects: Aculops lycopersici; Animals; Arthropods; Biology; Cyclopentanes - metabolism; DNA microarrays; Feeding; Gene expression; Genomics; Herbivores; Hormones; Host plants; Host-Parasite Interactions; Infestation; Jasmonic acid; Lycopersicon esculentum - genetics; Lycopersicon esculentum - immunology; Lycopersicon esculentum - parasitology; Mites; Mites - pathogenicity; Ontology; Oxylipins - metabolism; Pathogenesis; Plant Immunity; Population growth; Proteins; Salicylic acid; Tetranychus evansi; Tetranychus urticae; Tomatoes; Transcriptome
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms19103265

Tomato plants are attacked by diverse herbivorous arthropods, including by cell-content-feeding mites, such as the extreme generalist and specialists like and . Mite feeding induces plant defense responses that reduce mite performance. However, and suppress plant defenses via poorly understood mechanisms and, consequently, maintain a high performance on tomato. On a shared host, can be facilitated by either of the specialist mites, likely due to the suppression of plant defenses. To better understand defense suppression and indirect plant-mediated interactions between herbivorous mites, we used gene-expression microarrays to analyze the transcriptomic changes in tomato after attack by either a single mite species ( , , ) or two species simultaneously ( plus or plus ). Additionally, we assessed mite-induced changes in defense-associated phytohormones using LC-MS/MS. Compared to non-infested controls, jasmonates (JAs) and salicylate (SA) accumulated to higher amounts upon all mite-infestation treatments, but the response was attenuated after single infestations with defense-suppressors. Strikingly, whereas 8 to 10% of tomato genes were differentially expressed upon single infestations with or , respectively, only 0.1% was altered in -infested plants. Transcriptome analysis of dual-infested leaves revealed that primarily suppressed -induced JA defenses, while dampened -triggered host responses on a transcriptome-wide scale. The latter suggests that not solely down-regulates plant gene expression, but rather directs it back towards housekeeping levels. Our results provide valuable new insights into the mechanisms underlying host defense suppression and the plant-mediated facilitation of competing herbivores.