PSXII-21 CLA supplementation affects inflammatory and metabolic responses during an intramammary LPS challenge in early lactating dairy cows

Abstract We investigated the effects of CLA in early lactating dairy cows exposed to an intramammary LPS challenge. Multiparous Holstein cows (n=15) were assigned to a group with (CLA; n=8) and without CLA supplementation (CON; n=7) before parturition (CLA: 70 g/d lipid-encapsulated CLA (6.8 g t10,c...

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Published in:Journal of animal science 2018-12, Vol.96 (suppl_3), p.266-267
Main Authors: Gross, J, Grossen-Rösti, L, Heritier, R, Troescher, A, Bruckmaier, R
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container_issue suppl_3
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container_title Journal of animal science
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creator Gross, J
Grossen-Rösti, L
Heritier, R
Troescher, A
Bruckmaier, R
description Abstract We investigated the effects of CLA in early lactating dairy cows exposed to an intramammary LPS challenge. Multiparous Holstein cows (n=15) were assigned to a group with (CLA; n=8) and without CLA supplementation (CON; n=7) before parturition (CLA: 70 g/d lipid-encapsulated CLA (6.8 g t10,c12 and 6.6 g of c9,t11 CLA isomer), CON: 56 g/d control fat). An intramammary LPS challenge (50 μg LPS, E. coli O26:B6) was performed in wk 4 p.p.. Blood and milk were sampled every 30 and 60 min. Plasma was analyzed for glucose, free fatty acids (FFA), beta-hydroxybutyrate (BHB), cortisol, insulin, and glucagon. In milk, SCC and activity of lactate dehydrogenase (LDH) were determined. Metabolic, clinical, and endocrine responses were evaluated using mixed models with time, group and the time × group interaction as fixed effects and the individual cow as repeated subject. Differences between CLA and CON groups were assessed by Bonferroni corrected t-tests at P
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Multiparous Holstein cows (n=15) were assigned to a group with (CLA; n=8) and without CLA supplementation (CON; n=7) before parturition (CLA: 70 g/d lipid-encapsulated CLA (6.8 g t10,c12 and 6.6 g of c9,t11 CLA isomer), CON: 56 g/d control fat). An intramammary LPS challenge (50 μg LPS, E. coli O26:B6) was performed in wk 4 p.p.. Blood and milk were sampled every 30 and 60 min. Plasma was analyzed for glucose, free fatty acids (FFA), beta-hydroxybutyrate (BHB), cortisol, insulin, and glucagon. In milk, SCC and activity of lactate dehydrogenase (LDH) were determined. Metabolic, clinical, and endocrine responses were evaluated using mixed models with time, group and the time × group interaction as fixed effects and the individual cow as repeated subject. Differences between CLA and CON groups were assessed by Bonferroni corrected t-tests at P&lt;0.05. In wk 4 p.p., no differences in performance parameters were detected between CLA and CON, except for lower plasma glucose in CLA (P&lt;0.05). Between 2 and 3h after LPS stimulation, rectal temperature, plasma cortisol, LDH and SCC in milk began to rise. The increase of body temperature in CLA started earlier, the difference between peak and basal temperature was higher, and the decline thereafter occurred earlier in CLA compared to CON (P&lt;0.05). The increase in glucose following LPS stimulation was higher in CLA compared to CON (P&lt;0.05). Insulin started to increase after 3h relative to the LPS injection in CLA and CON (P=0.51). After 2h, BHB declined to a higher extent in CLA and approached CON from 5h onwards. In conclusion, CLA supplementation affected local and systemic immune responses. Cows supplemented with CLA provided more glucose and preferentially used BHB as an energy source.</description><identifier>ISSN: 0021-8812</identifier><identifier>EISSN: 1525-3163</identifier><identifier>DOI: 10.1093/jas/sky404.584</identifier><language>eng</language><publisher>US: Oxford University Press</publisher><subject>Abstracts</subject><ispartof>Journal of animal science, 2018-12, Vol.96 (suppl_3), p.266-267</ispartof><rights>The Author(s) 2018. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. 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Multiparous Holstein cows (n=15) were assigned to a group with (CLA; n=8) and without CLA supplementation (CON; n=7) before parturition (CLA: 70 g/d lipid-encapsulated CLA (6.8 g t10,c12 and 6.6 g of c9,t11 CLA isomer), CON: 56 g/d control fat). An intramammary LPS challenge (50 μg LPS, E. coli O26:B6) was performed in wk 4 p.p.. Blood and milk were sampled every 30 and 60 min. Plasma was analyzed for glucose, free fatty acids (FFA), beta-hydroxybutyrate (BHB), cortisol, insulin, and glucagon. In milk, SCC and activity of lactate dehydrogenase (LDH) were determined. Metabolic, clinical, and endocrine responses were evaluated using mixed models with time, group and the time × group interaction as fixed effects and the individual cow as repeated subject. Differences between CLA and CON groups were assessed by Bonferroni corrected t-tests at P&lt;0.05. In wk 4 p.p., no differences in performance parameters were detected between CLA and CON, except for lower plasma glucose in CLA (P&lt;0.05). Between 2 and 3h after LPS stimulation, rectal temperature, plasma cortisol, LDH and SCC in milk began to rise. The increase of body temperature in CLA started earlier, the difference between peak and basal temperature was higher, and the decline thereafter occurred earlier in CLA compared to CON (P&lt;0.05). The increase in glucose following LPS stimulation was higher in CLA compared to CON (P&lt;0.05). Insulin started to increase after 3h relative to the LPS injection in CLA and CON (P=0.51). After 2h, BHB declined to a higher extent in CLA and approached CON from 5h onwards. In conclusion, CLA supplementation affected local and systemic immune responses. 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Multiparous Holstein cows (n=15) were assigned to a group with (CLA; n=8) and without CLA supplementation (CON; n=7) before parturition (CLA: 70 g/d lipid-encapsulated CLA (6.8 g t10,c12 and 6.6 g of c9,t11 CLA isomer), CON: 56 g/d control fat). An intramammary LPS challenge (50 μg LPS, E. coli O26:B6) was performed in wk 4 p.p.. Blood and milk were sampled every 30 and 60 min. Plasma was analyzed for glucose, free fatty acids (FFA), beta-hydroxybutyrate (BHB), cortisol, insulin, and glucagon. In milk, SCC and activity of lactate dehydrogenase (LDH) were determined. Metabolic, clinical, and endocrine responses were evaluated using mixed models with time, group and the time × group interaction as fixed effects and the individual cow as repeated subject. Differences between CLA and CON groups were assessed by Bonferroni corrected t-tests at P&lt;0.05. In wk 4 p.p., no differences in performance parameters were detected between CLA and CON, except for lower plasma glucose in CLA (P&lt;0.05). Between 2 and 3h after LPS stimulation, rectal temperature, plasma cortisol, LDH and SCC in milk began to rise. The increase of body temperature in CLA started earlier, the difference between peak and basal temperature was higher, and the decline thereafter occurred earlier in CLA compared to CON (P&lt;0.05). The increase in glucose following LPS stimulation was higher in CLA compared to CON (P&lt;0.05). Insulin started to increase after 3h relative to the LPS injection in CLA and CON (P=0.51). After 2h, BHB declined to a higher extent in CLA and approached CON from 5h onwards. In conclusion, CLA supplementation affected local and systemic immune responses. 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title PSXII-21 CLA supplementation affects inflammatory and metabolic responses during an intramammary LPS challenge in early lactating dairy cows
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