Genomes of Escherichia coli bacteraemia isolates originating from urinary tract foci contain more virulence-associated genes than those from non-urinary foci and neutropaenic hosts

Escherichia coli is the leading cause of bacteraemia. In an era of emerging multi-drug-resistant strains, development of effective preventative strategies will be informed by knowledge of strain diversity associated with specific infective syndromes/patient groups. We hypothesised that the number of...

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Bibliographic Details
Published in:The Journal of infection 2018-12, Vol.77 (6), p.534-543
Main Authors: Dale, Adam P., Pandey, Anish K., Hesp, Richard J., Belogiannis, Konstantinos, Laver, Jay R., Shone, Clifford C., Read, Robert C.
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Antimicrobial resistance
Bacteraemia
Bacteremia - microbiology
Escherichia coli
Escherichia coli - genetics
Escherichia coli - isolation & purification
Escherichia coli - pathogenicity
Escherichia coli Infections - blood
Escherichia coli Infections - urine
ExPEC
Female
Genome, Bacterial
Humans
Immunocompetence
Male
Middle Aged
Neutropaenia
Neutropenia - microbiology
Phylogeny
Prospective Studies
Sequence Analysis, DNA
United Kingdom
Urinary Tract Infections - microbiology
Virulence
Virulence factor
Virulence Factors - genetics
Whole Genome Sequencing
Young Adult
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Summary:Escherichia coli is the leading cause of bacteraemia. In an era of emerging multi-drug-resistant strains, development of effective preventative strategies will be informed by knowledge of strain diversity associated with specific infective syndromes/patient groups. We hypothesised that the number of virulence factor (VF) genes amongst bacteraemia isolates from neutropaenic patients would be lower than isolates from immunocompetent patients. Immunocompetent and neutropaenic adults with E. coli bacteraemia were recruited prospectively and the source of bacteraemia determined. VF gene profiles were established in silico following whole genome sequencing. Isolates from individual patients were monoclonal. Strains from immunocompetent patients with urinary tract infective foci (UTIF) harboured more VF genes (median number of VF genes 16, range 8–24) than isolates from both immunocompetent patients with non-UTIF (10, 2–22, p = 0.0058) and neutropaenic patients with unknown focus of infection (NPUFI) (8, 3–13, p
ISSN:0163-4453
1532-2742
DOI:10.1016/j.jinf.2018.10.011