Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber

Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system 1 – 4 . Advances in wavefront-shaping methods and computational power have recently allowed for...

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Published in:Light, science & applications science & applications, 2018-12, Vol.7 (1), p.110-6, Article 110
Main Authors: Vasquez-Lopez, Sebastian A., Turcotte, Raphaël, Koren, Vadim, Plöschner, Martin, Padamsey, Zahid, Booth, Martin J., Čižmár, Tomáš, Emptage, Nigel J.
Format: Article
Language:English
Subjects:
639/624/1075/1076
639/624/1107/328
Applied and Technical Physics
Atomic
Calcium
Classical and Continuum Physics
Computational neuroscience
Dendrites
Diffraction
Lasers
Letter
Medical imaging
Molecular
Neuroimaging
Optical and Plasma Physics
Optical Devices
Optics
Photonics
Physics
Physics and Astronomy
Spatial discrimination
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Summary:Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system 1 – 4 . Advances in wavefront-shaping methods and computational power have recently allowed for a novel approach to high-resolution imaging, utilizing deterministic light propagation through optically complex media and, of particular importance for this work, multimode optical fibers (MMFs) 5 – 7 . We report a compact and highly optimized approach for minimally invasive in vivo brain imaging applications. The volume of tissue lesion was reduced by more than 100-fold, while preserving diffraction-limited imaging performance utilizing wavefront control of light propagation through a single 50-μm-core MMF. Here, we demonstrated high-resolution fluorescence imaging of subcellular neuronal structures, dendrites and synaptic specializations, in deep-brain regions of living mice, as well as monitored stimulus-driven functional Ca 2+ responses. These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage, heralding new possibilities for deep-brain imaging in vivo.
ISSN:2047-7538
2095-5545
2047-7538
DOI:10.1038/s41377-018-0111-0