C and N metabolism in barley leaves and peduncles modulates responsiveness to changing CO2

• Published in: Journal of experimental botany 2019-01, Vol.70 (2), p.599-611
• Main Authors: Torralbo, Fernando, Vicente, Rubén, Morcuende, Rosa, González-Murua, Carmen, Aranjuelo, Iker
• Format: Article
• Language: English
• Subjects: Carbon - metabolism; Carbon Dioxide - administration & dosage; Carbon Dioxide - metabolism; Climate Change; Hordeum - metabolism; Nitrogen - metabolism; Plant Leaves - metabolism; Research Papers
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/ery380

Barley plants with higher carbon sink capacity in the peduncle are able to maintain photosynthetic capacity by avoiding build-up of carbohydrates in the flag leaf. Abstract Balancing of leaf carbohydrates is a key process for maximising crop performance in elevated CO2 environments. With the aim of testing the role of the carbon sink-source relationship under different CO2 conditions, we performed two experiments with two barley genotypes (Harrington and RCSL-89) exposed to changing CO2. In Experiment 1, the genotypes were exposed to 400 and 700 ppm CO2. Elevated CO2 induced photosynthetic acclimation in Harrington that was linked with the depletion of Rubisco protein. In contrast, a higher peduncle carbohydrate-storage capacity in RSCL-89 was associated with a better balance of leaf carbohydrates that could help to maximize the photosynthetic capacity under elevated CO2. In Experiment 2, plants that were grown at 400 ppm or 700 ppm CO2 for 5 weeks were switched to 700 ppm or 400 ppm CO2, respectively. Raising CO2 to 700 ppm increased photosynthetic rates with a reduction in leaf carbohydrate content and an improvement in N assimilation. The increase in nitrate content was associated with up-regulation of genes of protein transcripts of photosynthesis and N assimilation that favoured plant performance under elevated CO2. Finally, decreasing the CO2 from 700 ppm to 400 ppm revealed that both stomatal closure and inhibited expression of light-harvesting proteins negatively affected photosynthetic performance and plant growth.