Magnitude of Vancomycin-Resistant Enterococci (VRE) Colonization among HIV-Infected Patients Attending ART Clinic in West Amhara Government Hospitals

• Published in: International journal of microbiology 2018-01, Vol.2018 (2018), p.1-7
• Main Authors: Yismaw, Gizachew, Derbie, Awoke, Abera, Bayeh, Agegne, Manamenot, Shiferaw, Melashu Balew
• Format: Article
• Language: English
• Subjects: Antibiotic resistance; Antibiotics; Antiinfectives and antibacterials; Antimicrobial agents; Antiretroviral drugs; Antiretroviral therapy; Colonization; Data collection; Data processing; Diabetes; Diffusion rate; Disease control; Drug resistance; Health sciences; Hemodialysis; HIV; Hospitals; Human immunodeficiency virus; Immunocompromised hosts; Infectious diseases; Intensive care; Intestine; Laboratories; Microbiology; Multidrug resistance; Nosocomial infection; Nosocomial infections; Patients; Plates (structural members); Regression analysis; Regression models; Sample size; Statistical analysis; Urinary tract diseases; Urinary tract infections; Urogenital system; Vancomycin
• ISSN: 1687-918X; 1687-9198
• DOI: 10.1155/2018/7510157

Background. Enterococci that colonize the intestinal tract of immunocompromised patients are an important cause of nosocomial infections. Data on the prevalence of vancomycin-resistant Enterococci (VRE) and its antimicrobial susceptibility patterns and associated factors are scarce in the present study area. Therefore, this study was conducted aimed at determining the prevalence of VRE colonization among HIV-infected patients attending ART clinic at West Amhara Government Hospitals. Methods. A cross-sectional study was conducted from 1 February 2017 to 31 May 2017. A total of 349 HIV patients were included in the study. A pretested structured questionnaire was used to collect sociodemographic data and possible associated factors for VRE colonization. Identification and confirmation of Enterococci from stool sample was performed based on the standard procedures. Antimicrobial susceptibility testing was done using the Kirby–Bauer disk diffusion method on the Muller–Hinton agar plate as per the standard protocol, and resistance profile of the isolates was determined according to Clinical and Laboratory Standards Institute (CLIS). Data were analyzed using SPSS v23. Descriptive analysis was used to visualize differences within data. Moreover, the stepwise logistic regression model was done to assess factors associated with VRE colonization. P value was set at 0.05 to indicate statistical significance difference. Results. The overall colonization status of Enterococci was at 63% (220/349). The VRE colonization was at 17 (7.7% (95% CI: 4.9–12.0)). Among Enterococcal isolates tested for antimicrobial susceptibility, 142 (64.5%) were found resistant to two or more antibiotics. Antibiotic treatment (for >2 weeks) and history of hospital admission in the last six month were found statistically associated for VRE colonization (AOR = 10.18, (95% CI: 1.9–53.20)) and (AOR = 20.17; (95% CI: 5.22–77.93)), respectively. Conclusions. The observed VRE with multidrug resistance colonization need a periodic surveillance of antimicrobial testing to detect emerging resistance and prevent the spread of further drug resistance.