Leukocyte expression profiles reveal gene sets with prognostic value for seizure-free outcome following stereotactic laser amygdalohippocampotomy

Among patients with intractable epilepsy, the most commonly performed surgical procedure is craniotomy for amygdalohippocampectomy (AH). Stereotactic laser amygdalohippocampotomy (SLAH) has also been recently employed as a minimally invasive treatment for intractable temporal lobe epilepsy (TLE). Am...

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Bibliographic Details
Published in:Scientific reports 2019-01, Vol.9 (1), p.1086-1086, Article 1086
Main Authors: Sprissler, Ryan, Bina, Robert, Kasoff, Willard, Witte, Marlys H., Bernas, Michael, Walter, Christina, Labiner, David M., Lau, Branden, Hammer, Michael F., Weinand, Martin E.
Format: Article
Language:English
Subjects:
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Astrocytes
Convulsions & seizures
Epilepsy
Humanities and Social Sciences
Inflammation
Lipid metabolism
Multidimensional scaling
multidisciplinary
Oligodendrocytes
Patients
Ribonucleic acid
RNA
Scaling
Science
Science (multidisciplinary)
Seizures
Temporal lobe
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Summary:Among patients with intractable epilepsy, the most commonly performed surgical procedure is craniotomy for amygdalohippocampectomy (AH). Stereotactic laser amygdalohippocampotomy (SLAH) has also been recently employed as a minimally invasive treatment for intractable temporal lobe epilepsy (TLE). Among patients treated with AH and SLAH approximately 65% and 54% of patients become seizure-free, respectively. Therefore, selection criteria for surgical candidates with improved prognostic value for post-operative seizure-free outcome are greatly needed. In this study, we perform RNA sequencing (RNA-Seq) on whole blood leukocyte samples taken from 16 patients with intractable TLE prior to SLAH to test the hypothesis that pre-operative leukocyte RNA expression profiles are prognostic for post-operative seizure outcome. Multidimensional scaling analysis of the RNA expression data indicated separate clustering of patients with seizure free (SF) and non-seizure-free (NSF) outcomes. Differential expression (DE) analysis performed on SF versus NSF groups revealed 24 significantly differentially expressed genes (≥2.0-fold change, p-value 
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-37763-5