Genome-Wide Identification of Cyclophilin Gene Family in Cotton and Expression Analysis of the Fibre Development in Gossypium barbadense

• Published in: International journal of molecular sciences 2019-01, Vol.20 (2), p.349
• Main Authors: Chen, Qin, Chen, Quan-Jia, Sun, Guo-Qing, Zheng, Kai, Yao, Zheng-Pei, Han, Yu-Hui, Wang, Li-Ping, Duan, Ya-Jie, Yu, Dao-Qian, Qu, Yan-Ying
• Format: Article
• Language: English
• Subjects: Abiotic stress; Brassica rapa; Chitinase; Chromosomes; Chromosomes, Plant - genetics; Cotton; Cotton Fiber; Cotton fibers; Cultivars; Cyclophilins - genetics; Elongation; Gene expression; Gene Expression Regulation, Plant; Genes; Genes, Plant; Genome, Plant; Genomes; Gossypium - genetics; Gossypium - growth & development; Gossypium barbadense; Homeostasis; Host plants; Identification; Low temperature; miRNA; Molecular weight; Multigene Family; Mutants; Phylogenetics; Phylogeny; Plant Proteins - genetics; Plant Proteins - metabolism; Promoter Regions, Genetic - genetics; Proteins; Signal transduction; Species Specificity; Virulence
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20020349

Cyclophilins (CYPs) are a member of the immunophilin superfamily (in addition to FKBPs and parvulins) and play a significant role in peptidyl-prolyl - isomerase (PPIase) activity. Previous studies have shown that CYPs have important functions in plants, but no genome-wide analysis of the cotton gene family has been reported, and the specific biological function of this gene is still elusive. Based on the release of the cotton genome sequence, we identified 75, 78, 40 and 38 gene sequences from , , , and , respectively; 221 genes were unequally located on chromosomes. Phylogenetic analysis showed that 231 genes clustered into three major groups and eight subgroups. Collinearity analysis showed that segmental duplications played a significant role in the expansion of members in cotton. There were light-responsiveness, abiotic-stress and hormone-response elements upstream of most of the CYPs. In addition, the motif composition analysis revealed that 49 cyclophilin proteins had extra domains, including TPR (tetratricopeptide repeat), coiled coil, U-box, RRM (RNA recognition motif), WD40 (RNA recognition motif) and zinc finger domains, along with the cyclophilin-like domain (CLD). The expression patterns based on qRT-PCR showed that six expression levels showed greater differences between Xinhai21 (long fibres, ) and Ashmon (short fibres, ) at 10 and 20 days postanthesis (DPA). These results signified that genes are involved in the elongation stage of cotton fibre development. This study provides a valuable resource for further investigations of gene functions and molecular mechanisms in cotton.