Single-stranded DNA and RNA origami

Self-folding of an information-carrying polymer into a defined structure is foundational to biology and offers attractive potential as a synthetic strategy. Although multicomponent self-assembly has produced complex synthetic nanostructures, unimolecular folding has seen limited progress. We describ...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2017-12, Vol.358 (6369), p.1402-1402
Main Authors: Han, Dongran, Qi, Xiaodong, Myhrvold, Cameron, Wang, Bei, Dai, Mingjie, Jiang, Shuoxing, Bates, Maxwell, Liu, Yan, An, Byoungkwon, Zhang, Fei, Yan, Hao, Yin, Peng
Format: Article
Language:English
Subjects:
Biology
Cells (biology)
Codification
Cohesion
Complexity
Crossovers
Deoxyribonucleic acid
Design
DNA
DNA Replication
DNA structure
DNA, Single-Stranded - chemistry
DNA, Single-Stranded - genetics
Double-stranded RNA
Escherichia coli
Folding
Innovations
Knots
Macromolecules
Nanostructure
Nanostructures - chemistry
Nanotechnology
Nanotechnology - methods
Nucleic acids
Nucleotides
Nucleotides - chemistry
Polymers
Protein folding
Proteins
Replication
RESEARCH ARTICLE SUMMARY
Ribonucleic acid
RNA
RNA - chemistry
RNA Folding
Self-assembly
Single-stranded DNA
Strands
Strategy
Structure-function relationships
Synthesis
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Summary:Self-folding of an information-carrying polymer into a defined structure is foundational to biology and offers attractive potential as a synthetic strategy. Although multicomponent self-assembly has produced complex synthetic nanostructures, unimolecular folding has seen limited progress. We describe a framework to design and synthesize a single DNA or RNA strand to self-fold into a complex yet unknotted structure that approximates an arbitrary user-prescribed shape. We experimentally construct diverse multikilobase single-stranded structures, including a ~10,000-nucleotide (nt) DNA structure and a ~6000-nt RNA structure. We demonstrate facile replication of the strand in vitro and in living cells. The work here thus establishes unimolecular folding as a general strategy for constructing complex and replicable nucleic acid nanostructures, and expands the design space and material scalability for bottom-up nanotechnology.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.aao2648