RNA Polymerase Clamp Movement Aids Dissociation from DNA but Is Not Required for RNA Release at Intrinsic Terminators

• Published in: Journal of molecular biology 2019-02, Vol.431 (4), p.696-713
• Main Authors: Bellecourt, Michael J., Ray-Soni, Ananya, Harwig, Alex, Mooney, Rachel Anne, Landick, Robert
• Format: Article
• Language: English
• Subjects: DNA - metabolism; DNA-Directed RNA Polymerases - metabolism; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Nucleic Acid Conformation; Nucleic Acids - metabolism; Nucleotides - metabolism; RNA - metabolism; termination commitment; Terminator Regions, Genetic - genetics; transcription elongation; transcription pausing; Transcription, Genetic - physiology; trigger loop
• ISSN: 0022-2836; 1089-8638
• DOI: 10.1016/j.jmb.2019.01.003

In bacteria, disassembly of elongating transcription complexes (ECs) can occur at intrinsic terminators in a 2- to 3-nucleotide window after transcription of multiple kilobase pairs of DNA. Intrinsic terminators trigger pausing on weak RNA–DNA hybrids followed by formation of a strong, GC-rich stem–loop in the RNA exit channel of RNA polymerase (RNAP), inactivating nucleotide addition and inducing dissociation of RNA and RNAP from DNA. Although the movements of RNA and DNA during intrinsic termination have been studied extensively leading to multiple models, the effects of RNAP conformational changes remain less well defined. RNAP contains a clamp domain that closes around the nucleic acid scaffold during transcription initiation and can be displaced by either swiveling or opening motions. Clamp opening is proposed to promote termination by releasing RNAP–nucleic acid contacts. We developed a cysteine crosslinking assay to constrain clamp movements and study effects on intrinsic termination. We found that biasing the clamp into different conformations perturbed termination efficiency, but that perturbations were due primarily to changes in elongation rate, not the competing rate at which ECs commit to termination. After commitment, however, inhibiting clamp movements slowed release of DNA but not of RNA from the EC. We also found that restricting trigger-loop movements with the RNAP inhibitor microcin J25 prior to commitment inhibits termination, in agreement with a recently proposed multistate–multipath model of intrinsic termination. Together our results support views that termination commitment and DNA release are separate steps and that RNAP may remain associated with DNA after termination. [Display omitted] •Disulfide bond crosslinks probe the role of the RNAP clamp domain in termination.•RNA but not DNA can release at terminators when the RNAP clamp is closed.•Restricting RNAP clamp movement affects elongation rate more than termination rate.•Inhibiting TL conformational flexibility impairs both RNA and DNA releases.