Persistence of Schistosoma japonicum DNA in a Kidney-Liver Transplant Recipient

Mitochondrial genome analysis of suggests that diversity of intermediate host snails drove intra-species divergence during its expansion in Asia. We applied the knowledge of this genomic variation to study an unusual patient we recently diagnosed with schistosomiasis. The patient had not visited any...

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Bibliographic Details
Published in:The American journal of tropical medicine and hygiene 2019-01, Vol.100 (3), p.584-587
Main Authors: Kron, Michael, Gordon, Catherine, Bauers, Timothy, Lu, Zouyan, Mahatme, Sheran, Shah, Janaki, Saeian, Kia, McManus, Donald P
Format: Article
Language:English
Subjects:
Adult
Animals
Anthelmintics - therapeutic use
Colon - parasitology
Deoxyribonucleic acid
DNA
DNA, Helminth - isolation & purification
Humans
Kidney Transplantation
Liver Transplantation
Liver transplants
Ovum
Phylogeny
Praziquantel - therapeutic use
Schistosoma japonicum - genetics
Transplant Recipients
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Summary:Mitochondrial genome analysis of suggests that diversity of intermediate host snails drove intra-species divergence during its expansion in Asia. We applied the knowledge of this genomic variation to study an unusual patient we recently diagnosed with schistosomiasis. The patient had not visited any schistosomiasis-endemic countries for more than 35 years and had no idea where she became infected. Unusual clinical features of this patient included the absence of egg granulomas in tissue and persistent noncalcified eggs despite multiple praziquantel (PZQ) treatments over 7 years. A digital droplet polymerase chair reaction (PCR) assay that specifically targets the schistosome 1,4 dihydronicotinamide adenine dinucleotide-1 (NADH1) dehydrogenase-1 mitochondrial gene successfully amplified parasite DNA extracted from colon biopsies. DNA sequence analysis of parasite DNA revealed that it was a Philippine strain of . Future molecular studies using stored DNA from patients such as this may provide new insight into why some persons do not respond well to PZQ treatment.
ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.18-0752