Integrative comparative analyses of metabolite and transcript profiles uncovers complex regulatory network in tomato (Solanum lycopersicum L.) fruit undergoing chilling injury

• Published in: Scientific reports 2019-03, Vol.9 (1), p.4470-4470, Article 4470
• Main Authors: Zhang, Wen-Fa, Gong, Ze-Hao, Wu, Meng-Bo, Chan, Helen, Yuan, Yu-Jin, Tang, Ning, Zhang, Qiang, Miao, Ming-Jun, Chang, Wei, Li, Zhi, Li, Zheng-Guo, Jin, Liang, Deng, Wei
• Format: Article
• Language: English
• Subjects: 101/58; 38/39; 38/91; 45/77; 631/449/1659; 631/449/2661; 96/47; Aconitic Acid - chemistry; Alanine; Alanine transaminase; Amino acids; ATP Citrate (pro-S)-Lyase - genetics; ATP citrate lyase; Chilling; Citric Acid - chemistry; Cold; Cold Temperature; Fruits; Gene Expression Profiling - methods; Gene Expression Regulation, Plant; Gene Regulatory Networks; Humanities and Social Sciences; Isocitrate dehydrogenase; Isocitrate Dehydrogenase - genetics; Leucine; Lycopersicon esculentum - chemistry; Lycopersicon esculentum - genetics; Metabolic Networks and Pathways; Metabolites; Metabolomics; Metabolomics - methods; Molecular modelling; multidisciplinary; Organic acids; Plant Proteins - genetics; Science; Science (multidisciplinary); Stress, Physiological; Succinic Acid - chemistry; Tomatoes; Transcription
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-41065-9

Tomato fruit are especially susceptible to chilling injury (CI) when continuously exposed to temperatures below 12 °C. In this study, integrative comparative analyses of transcriptomics and metabolomics data were performed to uncover the regulatory network in CI tomato fruit. Metabolite profiling analysis found that 7 amino acids, 27 organic acids, 16 of sugars and 22 other compounds had a significantly different content while transcriptomics data showed 1735 differentially expressed genes (DEGs) were down-regulated and 1369 were up-regulated in cold-stored fruit. We found that the contents of citrate, cis-aconitate and succinate were increased, which were consistent with the expression of ATP-citrate synthase ( ACS ) and isocitrate dehydrogenase ( IDH ) genes in cold-treated tomato fruit. Cold stress promotes the expression of ACS and IDH which may increase the synthesis of citrate, cis-aconitate and succinate. Alanine and leucine had increased contents, which may result from alanine aminotransferase ( ALT ) and branched-chain amino acid aminotransferase (BcAT )’s high expression levels, respectively. Overall the transcriptomics and metabolomics data in our study explain the molecular mechanisms of the chilling injury and expands our understanding of the complex regulatory mechanisms of a metabolic network in response to chilling injury in tomato fruit.