Treatment with eucalyptol mitigates cigarette smoke-induced lung injury through suppressing ICAM-1 gene expression

• Published in: Bioscience reports 2018-08, Vol.38 (4)
• Main Authors: Yu, Na, Sun, Yi-Tian, Su, Xin-Ming, He, Miao, Dai, Bing, Kang, Jian
• Format: Article
• Language: English
• Subjects: Animals; Anti-Infective Agents - therapeutic use; Cigarette Smoking - adverse effects; Down-Regulation - drug effects; Eucalyptol - therapeutic use; Intercellular Adhesion Molecule-1 - genetics; Lung Injury - drug therapy; Lung Injury - etiology; Lung Injury - genetics; Male; Pulmonary Disease, Chronic Obstructive - drug therapy; Pulmonary Disease, Chronic Obstructive - etiology; Pulmonary Disease, Chronic Obstructive - genetics; Rats, Sprague-Dawley; Tobacco Smoke Pollution - adverse effects
• ISSN: 0144-8463; 1573-4935
• DOI: 10.1042/BSR20171636

The present study was conducted to investigate the clinical significance of Eucalyptol in treating cigarette smoke-induced lung injury with the potential mechanism involved in the event. Rats were exposed to air (control) and cigarette smoke (smoking) after they were treated with Eucalyptol (260 mg/kg) orally once a day for 12 weeks. Cell counts of bronchoalveolar lavage fluid (BALF), measurements of mean liner intercept (MLI) and mean alveolar number (MAN), and lung function test were executed in experimental animals. Contents of cytokines and intercellular adhesion molecule (ICAM)-1 in BALF and ICAM-1 protein and mRNA expression in lung tissues were determined by ELISA, immunohistochemistry (IHC), and RT-PCR, respectively. A rat model of chronic obstructive pulmonary disease (COPD) displayed declining lung function, increased cell counts and cytokine production in BALF, and emphysema-like lesions in cigarette smoke-exposed lungs compared with the controls (all