Oral thermosensing by murine trigeminal neurons: modulation by capsaicin, menthol and mustard oil

• Published in: The Journal of physiology 2019-04, Vol.597 (7), p.2045-2061
• Main Authors: Leijon, Sara C. M., Neves, Amanda F., Breza, Joseph M., Simon, Sidney A., Chaudhari, Nirupa, Roper, Stephen D.
• Format: Article
• Language: English
• Subjects: Allyl isothiocyanate; Animals; Calcium imaging; Calcium-Binding Proteins - metabolism; Capsaicin; Capsaicin - pharmacology; chemesthesis; Cold Temperature; Female; Ganglia; Green Fluorescent Proteins; Hot Temperature; Isothiocyanate; Male; Menthol; Menthol - pharmacology; Mice; Mustard Plant; Neuromodulation; Neurons; Neurons - drug effects; Neuroscience; Nociceptors; Pain perception; Plant Oils - pharmacology; Research Paper; sensory neurons; Spices; thermosensation; Thermosensing - physiology; Transient Receptor Potential Channels - physiology; Trigeminal ganglion; Trigeminal Nerve - cytology
• ISSN: 0022-3751; 1469-7793
• DOI: 10.1113/JP277385

Key points Orosensory thermal trigeminal afferent neurons respond to cool, warm, and nociceptive hot temperatures with the majority activated in the cool range. Many of these thermosensitive trigeminal orosensory afferent neurons also respond to capsaicin, menthol, and/or mustard oil (allyl isothiocyanate) at concentrations found in foods and spices. There is significant but incomplete overlap between afferent trigeminal neurons that respond to oral thermal stimulation and to the above chemesthetic compounds. Capsaicin sensitizes warm trigeminal thermoreceptors and orosensory nociceptors; menthol attenuates cool thermoresponses. When consumed with foods, mint, mustard, and chili peppers generate pronounced oral thermosensations. Here we recorded responses in mouse trigeminal ganglion neurons to investigate interactions between thermal sensing and the active ingredients of these plants – menthol, allyl isothiocyanate (AITC), and capsaicin, respectively – at concentrations found in foods and commercial hygiene products. We carried out in vivo confocal calcium imaging of trigeminal ganglia in which neurons express GCaMP3 or GCAMP6s and recorded their responses to oral stimulation with thermal and the above chemesthetic stimuli. In the V3 (oral sensory) region of the ganglion, thermoreceptive neurons accounted for ∼10% of imaged neurons. We categorized them into three distinct classes: cool‐responsive and warm‐responsive thermosensors, and nociceptors (responsive only to temperatures ≥43–45 °C). Menthol, AITC, and capsaicin also elicited robust calcium responses that differed markedly in their latencies and durations. Most of the neurons that responded to these chemesthetic stimuli were also thermosensitive. Capsaicin and AITC increased the numbers of warm‐responding neurons and shifted the nociceptor threshold to lower temperatures. Menthol attenuated the responses in all classes of thermoreceptors. Our data show that while individual neurons may respond to a narrow temperature range (or even bimodally), taken collectively, the population is able to report on graded changes of temperature. Our findings also substantiate an explanation for the thermal sensations experienced when one consumes pungent spices or mint. Key points Orosensory thermal trigeminal afferent neurons respond to cool, warm, and nociceptive hot temperatures with the majority activated in the cool range. Many of these thermosensitive trigeminal orosensory afferent neurons also respond to cap