Optical intrinsic signal imaging with optogenetics reveals functional cortico-cortical connectivity at the columnar level in living macaques

Despite extensive research on primate cognitive function, understanding how anatomical connectivity at a neural circuit level relates to information transformation across different cortical areas remains primitive. New technology is needed to visualize inter-areal anatomical connectivity in living m...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2019-04, Vol.9 (1), p.6466, Article 6466
Main Authors: Nakamichi, Yu, Okubo, Kai, Sato, Takayuki, Hashimoto, Mitsuhiro, Tanifuji, Manabu
Format: Article
Language:English
Subjects:
13/44
631/378/2613/1875
631/378/2649
631/378/3920
Animals
Cerebral cortex
Cerebral Cortex - diagnostic imaging
Cerebral Cortex - metabolism
Cerebral hemispheres
Channelrhodopsins - biosynthesis
Cognitive ability
Connectome
Functional magnetic resonance imaging
Genetics
Humanities and Social Sciences
Information processing
Macaca mulatta
Magnetic Resonance Imaging
Male
multidisciplinary
Neural networks
Neurons - cytology
Neurons - metabolism
Optical Imaging
Optics
Optogenetics
Science
Science (multidisciplinary)
Structure-function relationships
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Despite extensive research on primate cognitive function, understanding how anatomical connectivity at a neural circuit level relates to information transformation across different cortical areas remains primitive. New technology is needed to visualize inter-areal anatomical connectivity in living monkeys and to tie this directly to neurophysiological function. Here, we developed a novel method to investigate this structure-function relationship, by combining optical intrinsic signal imaging (OISI) with optogenetic stimulation in living monkeys (opto-OISI). The method involves expressing channelrhodophsin-2 in one area (source) followed by optical imaging of optogenetic activations in the other area (target). We successfully demonstrated the potential of the method with interhemispheric columnar projection patterns between V1/V2 border regions. Unlike the combination of optogenetics and functional magnetic resonance imaging (opto-fMRI), opto-OISI has the advantage of enabling us to detect responses of small clusters of neurons, even if the clusters are sparsely distributed. We suggest that opto-OISI can be a powerful approach to understanding cognitive function at the neural circuit level, directly linking inter-areal circuitry to fine-scale structure and function.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-42923-2