Light Modulates the Physiology of Nonphototrophic Actinobacteria

• Published in: Journal of bacteriology 2019-05, Vol.201 (10), p.1
• Main Authors: Maresca, Julia A, Keffer, Jessica L, Hempel, Priscilla P, Polson, Shawn W, Shevchenko, Olga, Bhavsar, Jaysheel, Powell, Deborah, Miller, Kelsey J, Singh, Archana, Hahn, Martin W
• Format: Article
• Language: English
• Subjects: Actinobacteria; Actinobacteria - growth & development; Actinobacteria - metabolism; Actinobacteria - radiation effects; Aquatic environment; Bacterial Proteins - metabolism; Bacteriology; Carbohydrate Metabolism - radiation effects; Carbohydrates; Conversion; Cryptochromes - metabolism; Gene Expression Regulation, Bacterial - radiation effects; Genomes; Growth rate; Light; Metabolism; Organic carbon; Phenotypes; Sugar; Transport
• ISSN: 0021-9193; 1098-5530
• DOI: 10.1128/JB.00740-18

Light is a source of energy and an environmental cue that is available in excess in most surface environments. In prokaryotic systems, conversion of light to energy by photoautotrophs and photoheterotrophs is well understood, but the conversion of light to information and the cellular response to that information have been characterized in only a few species. Our goal was to explore the response of freshwater , which are ubiquitous in illuminated aquatic environments, to light. We found that without functional photosystems grow faster in the light, likely because sugar transport and metabolism are upregulated in the light. Based on the action spectrum of the growth effect and comparisons of the genomes of three with this growth rate phenotype, we propose that the photosensor in these strains is a putative CryB-type cryptochrome. The ability to sense light and upregulate carbohydrate transport during the day could allow these cells to coordinate their time of maximum organic carbon uptake with the time of maximum organic carbon release by primary producers. Sunlight provides information about both place and time. In sunlit aquatic environments, primary producers release organic carbon and nitrogen along with other growth factors during the day. The ability of to coordinate organic carbon uptake and utilization with production of photosynthate enables them to grow more efficiently in the daytime, and it potentially gives them a competitive advantage over heterotrophs that constitutively produce carbohydrate transporters, which is energetically costly, or produce transporters only after detection of the substrate(s), which delays their response. Understanding how light cues the transport of organic carbon and its conversion to biomass is key to understanding biochemical mechanisms within the carbon cycle, the fluxes through it, and the variety of mechanisms by which light enhances growth.