Epigenetic Field Cancerization in Gastric Cancer: microRNAs as Promising Biomarkers

The biological role of microRNAs (miRNAs) in field cancerization is unknown. To investigate the involvement of miRNAs in gastric field cancerization, we evaluated the expression profile of ten miRNAs and their diagnostic value. We used three groups of FFPE gastric samples: non-cancer (NC), cancer ad...

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Published in:Journal of Cancer 2019-01, Vol.10 (6), p.1560-1569
Main Authors: Pereira, Adenilson Leão, Magalhães, Leandro, Moreira, Fabiano Cordeiro, Reis-das-Mercês, Laís, Vidal, Amanda Ferreira, Ribeiro-Dos-Santos, André Maurício, Demachki, Samia, Anaissi, Ana Karyssa Mendes, Burbano, Rommel Mario Rodríguez, Albuquerque, Paulo, Dos Santos, Sidney Emanuel Batista, de Assumpção, Paulo Pimentel, Ribeiro-Dos-Santos, Ândrea Kely Campos
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Language:English
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Research Paper
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Summary:The biological role of microRNAs (miRNAs) in field cancerization is unknown. To investigate the involvement of miRNAs in gastric field cancerization, we evaluated the expression profile of ten miRNAs and their diagnostic value. We used three groups of FFPE gastric samples: non-cancer (NC), cancer adjacent (ADJ) and gastric cancer (GC). The expression profiles of , , , , , , and were investigated using qRT-PCR. The results obtained by qRT-PCR were validated in Small RNA-Seq data from the TCGA database. The search for target genes of the studied miRNAs was performed in the miRTarBase public database and miRTargetLink tool, using experimentally validated interactions. In addition, we also performed the functional analysis of these genes using enrichment in KEGG pathways. The potential as biomarker was evaluated using a receiver operating characteristic (ROC) curve and the derived area under the curve (AUC>0.85) analysis. The miRNAs , , , , , , , and were up-regulated in ADJ and GC compared to NC (
ISSN:1837-9664
1837-9664
DOI:10.7150/jca.27457